Jeong Eun Seon, Son Han Am, Kim Min Kyung, Park Kyoung-Ho, Kay Sechan, Chae Pil Seok, Kim Jin Woong
Department of Bionano Technology, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea.
Korea Institute of Geoscience and Mineral Resources, Daejeon 305-350, Republic of Korea.
Colloids Surf B Biointerfaces. 2014 Nov 1;123:339-44. doi: 10.1016/j.colsurfb.2014.09.039. Epub 2014 Sep 28.
This study introduces a drop-based microfluidic approach to physically immobilize liposomes in microgel (liposomes-in-microgel) particles. For this, we generate a uniform liposomes-in-water-in-oil emulsion in a capillary-based microfluidic device. Basically, we have investigated how the flow rate and flow composition affect generation of emulsion precursor drops in micro-channels. Then, the precursor emulsion drops are solidified by photo-polymerization. From characterization of hydrogel mesh sizes, we have figured out that the mesh size of the liposomes-in-microgel particles is bigger than that of bare microgel particles, since liposomes take space in the hydrogel phase. In our further study on drug releasing, we have observed that immobilization of liposomes in the microgel particles can not only remarkably retard drug releasing, but also enables a sustained release, which stems from the enhanced matrix viscosity of the surrounding hydrogel phase.
本研究介绍了一种基于液滴的微流控方法，用于将脂质体物理固定在微凝胶（微凝胶包裹脂质体）颗粒中。为此，我们在基于毛细管的微流控装置中生成了均匀的水包油包水型乳液。基本上，我们研究了流速和流动组成如何影响微通道中乳液前驱体液滴的生成。然后，通过光聚合使前驱体乳液液滴固化。通过对水凝胶网孔尺寸的表征，我们发现微凝胶包裹脂质体颗粒的网孔尺寸比裸微凝胶颗粒的大，因为脂质体在水凝胶相中占据空间。在我们进一步的药物释放研究中，我们观察到脂质体固定在微凝胶颗粒中不仅能显著延缓药物释放，还能实现持续释放，这源于周围水凝胶相基质粘度的增加。
