Yang Chih-Hui, Lin Yung-Sheng, Huang Keng-Shiang, Huang Yu-Che, Wang Eng-Chi, Jhong Jia-Ying, Kuo Chun-Yen
Department of Biological Science & Technology, I-Shou University, Taiwan.
Lab Chip. 2009 Jan 7;9(1):145-50. doi: 10.1039/b807454b. Epub 2008 Oct 21.
A microfluidic device for generating monodisperse chitosan microparticles and separating the desired particle from smaller particles created as a byproduct of this process was described. The purpose of this study is to separate the satellite droplets from their parent droplets to enhance the size uniformity of the desired microparticles. A double T-junction design was first employed to control the emulsification and the separation, respectively. The results show that the size and gap of the parent droplets are tunable by adjusting the water and oil flow rates. A separation ratio of the satellite droplets of more than 99% was observed. The proposed microfluidic chip is capable of generating relatively uniform micro-droplets with well controllable diameter, and it has the added advantages of being a simple, low cost, and high throughput process. In the future this apparatus can be used to fabricate size-controlled monodisperse microparticles to act as drug carriers for biotechnology and biomedicine applications.
本文描述了一种微流控装置，用于生成单分散壳聚糖微粒，并从该过程作为副产物产生的较小颗粒中分离出所需颗粒。本研究的目的是将卫星液滴与其母液滴分离，以提高所需微粒的尺寸均匀性。首先采用双T型结设计分别控制乳化和分离过程。结果表明，通过调节水和油的流速，可以调节母液滴的尺寸和间距。观察到卫星液滴的分离率超过99%。所提出的微流控芯片能够生成直径可控的相对均匀的微滴，并且具有简单、低成本和高通量的优点。未来，该装置可用于制造尺寸可控的单分散微粒，作为生物技术和生物医学应用中的药物载体。
