Johansson Fredrik, Dahlin Lars B
Department of Biology/Functional Zoology, Lund University, Sölvegatan 35, 223 62 Lund, Sweden.
Department of Clinical Sciences in Malmö/Hand Surgery, Lund University and Skåne University Hospital, Jan Waldenströms Gata 5, 205 02 Malmö, Sweden.
Biomed Res Int. 2014;2014:689127. doi: 10.1155/2014/689127. Epub 2014 Apr 17.
Multiple nerve branches were created during the regeneration procedure after a nerve injury and such multiple branches are suggested to be used to control, for example, prosthesis with many degrees of freedom. Transected rat sciatic nerve stumps were inserted into a nine mm long silicone tube, which contained four, five mm long, smaller tubes, thus leaving a five mm gap for regenerating nerve fibers. Six weeks later, several new nerve structures were formed not only in the four smaller tubes, but also in the spaces in-between. The 7-9 new continuous nerve structures, which were isolated as individual free nerves after removal of the tubes, were delineated by a perineurium and contained both myelinated and unmyelinated nerve fibers as well as blood vessels. Stimulation of the proximal nerve elicited contractions in distal muscles. Thin metal electrodes, inserted initially into the smaller tubes in some experiments, became embedded in the new nerve structures and when stimulated contractions of the distal muscles were observed. The "tubes within a tube" technique, creating multiple new nerves from a single "mother" nerve, can be used to record multiple signals for prosthetic device control or as sources for supply of multiple denervated targets.
在神经损伤后的再生过程中会形成多个神经分支，并且有人建议利用这些多个分支来控制例如具有多个自由度的假肢。将切断的大鼠坐骨神经残端插入一根9毫米长的硅胶管中，该硅胶管包含四根5毫米长的较小管子，从而为再生神经纤维留出5毫米的间隙。六周后，不仅在四根较小的管子中形成了几个新的神经结构，而且在它们之间的空间中也形成了新的神经结构。在移除管子后，将7 - 9个新的连续神经结构分离为单独的游离神经，这些神经结构由神经束膜界定，包含有髓鞘和无髓鞘神经纤维以及血管。刺激近端神经会引起远端肌肉收缩。在一些实验中，最初插入较小管子的细金属电极嵌入了新的神经结构中，当对其进行刺激时，观察到了远端肌肉的收缩。“管中管”技术能够从单一的“母”神经产生多个新神经，可用于记录多个信号以控制假肢装置，或作为多个失神经支配靶点的神经供应源。
