Heidary Masoumeh, Rakhshi Nahid, Pahlevan Kakhki Majid, Behmanesh Mehrdad, Sanati Mohammad Hossein, Sanadgol Nima, Kamaladini Hossein, Nikravesh Abbas
Department of Basic Sciences, Gonabad University of Medical Sciences, Gonabad, Islamic Republic of Iran.
Department of Molecular Sciences, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Islamic Republic of Iran.
J Neurol Sci. 2014 Aug 15;343(1-2):41-5. doi: 10.1016/j.jns.2014.05.013. Epub 2014 May 15.
IL-1B is released by monocytes, astrocytes and brain endothelial cells and seems to be involved in inflammatory reactions of the central nervous system (CNS) in multiple sclerosis (MS). This study aims to evaluate the expression level of IL-1B mRNA in peripheral blood mononuclear cells (PBMCs), genotype the rs16944 SNP and find out the role of this SNP on the expression level of IL-1B in MS patients. We found that the expression level of IL-1B in MS patients increased 3.336 times more than controls in PBMCs but the rs16944 SNP in the promoter region of IL-1B did not affect the expression level of this gene and there was not association of this SNP with MS in the examined population. Also, our data did not reveal any correlation between normalized expressions of IL-1B gene with age of participants, age of onset, and disease duration.
白细胞介素-1β（IL-1B）由单核细胞、星形胶质细胞和脑内皮细胞释放，似乎参与了多发性硬化症（MS）中枢神经系统（CNS）的炎症反应。本研究旨在评估外周血单核细胞（PBMCs）中IL-1B mRNA的表达水平，对rs16944单核苷酸多态性（SNP）进行基因分型，并探究该SNP对MS患者IL-1B表达水平的作用。我们发现，MS患者PBMCs中IL-1B的表达水平比对照组增加了3.336倍，但IL-1B启动子区域的rs16944 SNP并不影响该基因的表达水平，且在所检测人群中该SNP与MS无关联。此外，我们的数据未显示IL-1B基因的标准化表达与参与者年龄、发病年龄及病程之间存在任何相关性。
