Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi, Taiwan, ROC.
Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi, Taiwan, ROC.
Sci Total Environ. 2014 Aug 15;490:416-21. doi: 10.1016/j.scitotenv.2014.04.103. Epub 2014 May 24.
In this study, TaqMan fluorescent quantitative real-time PCR was performed to quantify Legionella species in reservoirs. Water samples were collected from 19 main reservoirs in Taiwan, and 12 (63.2%) were found to contain Legionella spp. The identified species included uncultured Legionella spp., L. pneumophila, L. jordanis, and L. drancourtii. The concentrations of Legionella spp. and L. pneumophila in the water samples were in the range of 1.8×10(2)-2.6×10(3) and 1.6×10(2)-2.4×10(2) cells/L, respectively. The presence and absence of Legionella spp. in the reservoir differed significantly in pH values. These results highlight the importance that L. pneumophila, L. jordanis, and L. drancourtii are potential pathogens in the reservoirs. The presence of L. pneumophila in reservoirs may be a potential public health concern that must be further examined.
在这项研究中，采用 TaqMan 荧光定量实时 PCR 对储水器中的军团菌属进行定量。从台湾的 19 个主要水库采集水样，发现 12 个（63.2%）含有军团菌属。鉴定出的物种包括未培养的军团菌属、嗜肺军团菌、L. jordanis 和 L. drancourtii。水样中军团菌属和嗜肺军团菌的浓度范围分别为 1.8×10(2)-2.6×10(3) 和 1.6×10(2)-2.4×10(2) 细胞/L。储水器中军团菌属的存在与否在 pH 值上有显著差异。这些结果强调了嗜肺军团菌、L. jordanis 和 L. drancourtii 在储水器中是潜在的病原体。军团菌属在储水器中的存在可能是一个潜在的公共卫生问题，需要进一步研究。
