Li Bo, Wang Zhijin, Cao Jun, Xing Chuanhong, Cui Liuxin
Wei Sheng Yan Jiu. 2014 Mar;43(2):213-8.
Evaluate the inherent toxicity of organic extracts in the effluents treated by present treatment and the new process find the scientific evidences of the new wastewater treatment technique to compare the removal efficiency of trace organic pollutants.
The Solid Phase Extraction was adopted to enrich the trace organic pollutants in the water samples with resin adsorbing, then detected the inherent toxicity of them by using Vicia faba root-tip micronucleus test and single cell gel electropheorsis test.
After concentrated 100 times, the organic extracts enriched from the water samples showed some positive reactions. The micronuclear rates of root-tip cells were of significantly difference between all groups (F = 42.526, P < 0.001). Compared with each other, the group of DMSO and the group of new technique were not significantly different from the negative control (P > 0.05), but the group of secondary effluent and the group of influent were significantly different from the new technique (P < 0.05), at last, there was significant difference between the secondary effluent and the influent (P < 0.05). After exposed with DMSO and the organic extracts, the length of comet tails and the tailing ratio were obviously different between all groups (F = 243.535 and 148.623 correspodingly, P < 0.001). Compared with negative control, the length of comet tails and the tailing ratio of the influent group and the secondary effluent group were significantly different (P < 0.01). Compared with the influent group, the comet tail length of the secondary effluent group and the tertiary effluent group were both shorter (P < 0.01), and the tailing ratio were both smaller (P < 0.01). The tailing ratio of the tertiary effluent group was significantly smaller than the secondary effluent group.
The inherent toxicity of urban sewage in Zhengzhou was significantly decreased after treated but it still possesses potential hazard to environment. And the trace organic pollutants in wastewater were removed by the new technique more efficiently than by the present treatment.
评估当前处理工艺和新工艺处理后废水中有机提取物的内在毒性，寻找新污水处理技术的科学依据，比较微量有机污染物的去除效率。
采用固相萃取法，用树脂吸附富集水样中的微量有机污染物，然后通过蚕豆根尖微核试验和单细胞凝胶电泳试验检测其内在毒性。
水样富集的有机提取物浓缩100倍后呈现一些阳性反应。各实验组根尖细胞微核率差异有统计学意义（F = 42.526，P < 0.001）。两两比较，二甲基亚砜组和新工艺组与阴性对照组差异无统计学意义（P > 0.05），但二级出水组和进水组与新工艺组差异有统计学意义（P < 0.05），最终，二级出水组与进水组差异有统计学意义（P < 0.05）。经二甲基亚砜和有机提取物处理后，各实验组彗星尾长和拖尾率差异有统计学意义（分别为F = 243.535和148.623，P < 0.001）。与阴性对照组比较，进水组和二级出水组彗星尾长和拖尾率差异有统计学意义（P < 0.01）。与进水组比较，二级出水组和三级出水组彗星尾长均较短（P < 0.01），拖尾率均较小（P < 0.01）。三级出水组拖尾率显著小于二级出水组。
郑州市城市污水经处理后内在毒性显著降低，但仍对环境存在潜在危害。新处理工艺对废水中微量有机污染物的去除效率高于现有处理工艺。
