Rawat Kamla, Solanki Pratima R, Arora Kavita, Bohidar H B
Special Centre for Nanosciences, Jawaharlal Nehru University, New Delhi, 110067, India.
Appl Biochem Biotechnol. 2014 Oct;174(3):1032-42. doi: 10.1007/s12010-014-0936-9. Epub 2014 May 29.
In this study, a very thin film of biocompatible gelatin B (GB) fabricated onto indium tin oxide (ITO)-coated glass substrate for electrochemical catalytic activity towards different metabolites has been investigated. The optical and electrochemical properties of bare GB/ITO electrode and with different metabolites were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and electrochemical techniques. The optical properties clearly indicate the structural and surface morphological changes on electrode surface. FTIR spectra showed displacement of the IR peaks towards smaller wave numbers, indicating possible existence of hydrogen bonding between the GB and metabolites. The catalytic behaviour of GB/ITO electrode towards ascorbic acid (AA), citric acid (CA), oxalic acid (OA), glucose (Glu), sucrose (Suc), lactose (Lac) and fructose (Fru) has been investigated by cyclic voltammetry (CV). The electrochemical response studies of GB/ITO electrode have been monitored with different metabolites in the range of 10-500 mg/dl. The sensitivity of GB/ITO electrode for AA and OA was found as 0.156 and 0.108 μA/(mg/dl cm(-2)) respectively. The results indicate that the GB/ITO electrode has higher specificity towards the AA and OA. The attractive properties of GB/ITO electrode provide the potential applications in the simultaneous detection of AA and OA. The excellent electrocatalytic behaviour of GB/ITO electrode may be useful towards the construction of electrochemical biosensors.
在本研究中，已对在氧化铟锡（ITO）涂层玻璃基板上制备的用于对不同代谢物具有电化学催化活性的非常薄的生物相容性明胶B（GB）薄膜进行了研究。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和电化学技术对裸GB/ITO电极以及与不同代谢物结合时的光学和电化学性质进行了表征。光学性质清楚地表明了电极表面的结构和表面形态变化。FTIR光谱显示红外峰向较小波数位移，表明GB与代谢物之间可能存在氢键。通过循环伏安法（CV）研究了GB/ITO电极对抗坏血酸（AA）、柠檬酸（CA）、草酸（OA）、葡萄糖（Glu）、蔗糖（Suc）、乳糖（Lac）和果糖（Fru）的催化行为。在10 - 500 mg/dl范围内，用不同代谢物监测了GB/ITO电极的电化学响应研究。发现GB/ITO电极对AA和OA的灵敏度分别为0.156和0.108 μA/(mg/dl cm⁻²)。结果表明，GB/ITO电极对AA和OA具有更高的特异性。GB/ITO电极的吸引人的性质为AA和OA的同时检测提供了潜在应用。GB/ITO电极优异的电催化行为可能对电化学生物传感器的构建有用。
