Marcelle C, Gale R P, Prokocimer M, Berrebi A, Merle-Beral H, Canaani E
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Genes Chromosomes Cancer. 1989 Nov;1(2):172-9. doi: 10.1002/gcc.2870010211.
The Philadelphia chromosome is present in more than 95% of chronic myelogenous leukemia patients and in up to 25% of patients with acute lymphocytic leukemia. The major consequence of the aberration is the fusion of the ABL and BCR genes. The position of the breakpoint on chromosome 22 determines which species of the potential three fused mRNAs and proteins will be synthesized. We have used the polymerase chain reaction (PCR) to detect these mRNAs in 53 patients and cell lines and found that around 20% contain simultaneously two BCR-ABL mRNAs, presumably due to a process of alternative splicing. The results also indicate that most patients in lymphocytic blast crisis of CML contain the mRNA in which bcr exon 2 is linked to ABL exon II. Finally, we identified, cloned, and characterized a BCR-related sequence that originated from mRNA.
费城染色体存在于超过95%的慢性粒细胞白血病患者以及高达25%的急性淋巴细胞白血病患者中。这种畸变的主要后果是ABL和BCR基因的融合。22号染色体上断点的位置决定了三种潜在融合mRNA和蛋白质中将合成哪一种。我们使用聚合酶链反应（PCR）在53例患者和细胞系中检测这些mRNA，发现约20%同时含有两种BCR-ABL mRNA，推测这是由于可变剪接过程所致。结果还表明，大多数处于慢性粒细胞白血病淋巴细胞急变期的患者含有bcr外显子2与ABL外显子II相连的mRNA。最后，我们鉴定、克隆并表征了一个源自mRNA的BCR相关序列。
