Korsak Jolanta, Goller Agnieszka, Rzeszotarska Agnieszka, Pleskacz Katarzyna
Cryo Letters. 2014 Jan-Feb;35(1):15-21.
Cryopreservaton of packed human red blood cells requires the use of cryoprotectants.
The study assessed physiological parameters of 40 RBC units frozen with either 40% glycerol or 6.7% HES.
After thawing, they were suspended in NaCl or in 6% HES. Tests of Hct, Hb, Na+ and K+ ions, ATP, 2,3-DPG, pH and erythrocyte stability were measured 30 minutes and 24 hours after thawing.
Hct was lower after thawing but did not differ significantly between two groups. Hb was lower after thawing, but was statistically significant higher in the HES group (43.8 g/unit vs 35.4 g/unit). K+ concentration increased after thawing and was significantly higher after 24 hours in the glycerol group (29.0 mEq/l vs 8.7 mEq/l). ATP concentration in the HES group was significantly lower (2.15 micromol/g) in comparison with the glycerol group (6.30 micromol/g) 24 hours after thawing. 2,3-DPG levels did not differ significantly between the methods. Stability of RBCs frozen in glycerol were better (94.58%) than RBCs frozen in HES (80.75%) measured 24 hours after thawing. ATP is better protected in erythrocytes frozen in glycerol than in HES.
Erythrocytes frozen with HES preserved more hemoglobin than with glycerol. Membrane permeability for Na+ and K+ ions was preserved better with HES. HES compared to glycerol offered better protection for erythrocytes.
人浓缩红细胞的冷冻保存需要使用冷冻保护剂。
本研究评估了用40%甘油或6.7%羟乙基淀粉（HES）冷冻的40个红细胞单位的生理参数。
解冻后，将它们悬浮于氯化钠或6%羟乙基淀粉中。在解冻后30分钟和24小时测量血细胞比容（Hct）、血红蛋白（Hb）、钠（Na+）和钾（K+）离子、三磷酸腺苷（ATP）、2,3-二磷酸甘油酸（2,3-DPG）、pH值及红细胞稳定性。
解冻后血细胞比容降低，但两组间无显著差异。解冻后血红蛋白降低，但羟乙基淀粉组在统计学上显著更高（43.8克/单位 vs 35.4克/单位）。解冻后钾离子浓度升高，甘油组在24小时后显著更高（29.0毫当量/升 vs 8.7毫当量/升）。解冻24小时后，羟乙基淀粉组的三磷酸腺苷浓度显著低于甘油组（2.15微摩尔/克 vs 6.30微摩尔/克）。两种方法间2,3-二磷酸甘油酸水平无显著差异。解冻24小时后测量，甘油冷冻的红细胞稳定性更好（94.58%），优于羟乙基淀粉冷冻的红细胞（80.75%）三磷酸腺苷在甘油冷冻的红细胞中比在羟乙基淀粉冷冻的红细胞中得到更好的保护。
与甘油相比，羟乙基淀粉冷冻的红细胞保留了更多血红蛋白。羟乙基淀粉对钠和钾离子的膜通透性保留更好。与甘油相比，羟乙基淀粉为红细胞提供了更好的保护。
