Rittmeyer I C, Nydegger U E
Central Laboratory of Haematology, University of Berne, Switzerland.
Vox Sang. 1992;62(3):141-5. doi: 10.1111/j.1423-0410.1992.tb01187.x.
Because hydroxyethyl starch (HAES) is used for volume replacement therapy and as a cryoprotectant for frozen red blood cells (RBCs), this compound, in contrast to glycerol, does not require labor-intensive removal from thawed cells prior to transfusion. We here report the effect of both glycerol and HAES on the RBC organic phosphates ATP and 2,3-diphosphoglyceric acid (2,3-DPG). The CPD-A1-stabilized RBCs of 20 healthy donors (3 females, 17 males) were separately frozen in either 40% glycerol or 6% HAES, of molecular weight 200,000. ATP and 2,3-DPG concentrations were determined in CPD-A1 RBCs before addition of cryoprotectant and in cryopreserved thawed RBCs after 24 h storage at -80 degrees C (glycerol) and -196 degrees C (HAES). It appears that HAES, but not glycerol, significantly reduces ATP concentrations whereas both lead to a reduction of 2,3-DPG concentrations; this reduction was more pronounced with glycerol than with HAES. Experiments with the blood of 6 donors demonstrated that HAES affects autohemolysis by 16%, in contrast to glycerol, after which cryoprotectant autohemolysis was affected by 3.1% only. RBC recoveries were comparable using glycerol or HAES as cryoprotectants. A distinct pattern of reduction of 2,3-DPG levels by glycerol and less by HAES, and of ATP levels by HAES but not by glycerol, emerges. Our findings may be of importance if HAES is to be introduced as a convenient cryoprotectant.
由于羟乙基淀粉（HAES）用于容量替代治疗并作为冷冻红细胞（RBC）的冷冻保护剂，与甘油相比，这种化合物在输血前无需从解冻的细胞中进行费力的去除。我们在此报告甘油和HAES对RBC有机磷酸盐ATP和2,3 - 二磷酸甘油酸（2,3 - DPG）的影响。20名健康供者（3名女性，17名男性）的CPD - A1稳定化RBC分别在40%甘油或6%分子量为200,000的HAES中冷冻。在添加冷冻保护剂之前测定CPD - A1 RBC中的ATP和2,3 - DPG浓度，并在-80℃（甘油）和-196℃（HAES）储存24小时后测定冷冻保存的解冻RBC中的浓度。似乎HAES而非甘油能显著降低ATP浓度，而两者都会导致2,3 - DPG浓度降低；甘油导致的这种降低比HAES更明显。对6名供者血液的实验表明，与甘油相比，HAES使自身溶血率增加16%，而甘油仅使冷冻保护剂自身溶血率增加3.1%。使用甘油或HAES作为冷冻保护剂时，RBC回收率相当。甘油和HAES对2,3 - DPG水平的降低模式不同，甘油降低得多，HAES降低得少；对ATP水平的影响也不同，HAES能降低，甘油则不能。如果要将HAES作为一种方便的冷冻保护剂引入，我们的发现可能具有重要意义。
