Huang Yi-Ming, Xia Ji-Yi, Jiang Rui
Zhonghua Nan Ke Xue. 2014 Apr;20(4):299-303.
To investigate the impact of hyperglycemia on the hydrogen sulfide (H2S) signaling pathway in rat penile tissue and its relationship with erectile function.
Twenty healthy male Sprague Dawley (SD) rats aged 8 weeks were randomly divided into groups A (4-week healthy control), B (4-week diabetes mellitus model), C (6-week healthy control) and D (6-week diabetes mellitus model). The rats in groups B and D were injected intraperitoneally with streptozotocin at 50 mg/kg to induce diabetes mellitus, while those in groups A and C with the same volume of normal saline. The animals were killed at 4 (groups A and B) and 6 weeks (groups C and D) after treatment for measurement of the maximal intracavernous pressure/mean arterial blood pressure (ICP(max)/MAP) by electrostimulation, determination of the H2S concentration in the plasma and penile tissue, and detection of the expressions of cystathionine-beta-synthetase (CBS) and cystathionine-gamma-lyase (CSE) in the penile corpus cavernosum by immunohisto- chemistry and Western blot.
With electrostimulation of the pelvic ganglia at 5V and 7 V, ICP(max)/MAP was significantly reduced in groups B (0.19 +/- 0.03 and 0.29 +/- 0.04) and D (0.14 +/- 0.04 and 0.25 +/- 0.04) as compared with A (0.46 +/- 0.07 and 0.68 +/- 0.09) and C (0.43 +/- 0.07 and 0.65 +/- 0.16) (P < 0.05). No statistically significant differences were found in the level of serum testosterone either between groups A and B ([469.19 +/- 126.46] ng/dl vs [359.08 +/- 60.06] ng/dl, P > 0.05) or between C and D ([470.44 +/- 209.28] ng/dl vs [297.01 +/- 96.58] ng/dl, P > 0.05). Groups B and D showed remarkable reduction in the H2S concentration (P < 0.05) and the expressions of CBS and CSE (P < 0.05) in comparison with A and C, and the CBS and CSE expressions were even more significantly decreased in D than in B (P < 0.05).
The reduced concentration of H2S and decreased expressions of CBS and CSE in the penile corpus cavernosum of the diabetic rats suggested that the H2S signaling pathway might be involved in hyperglycemia-induced erectile dysfunction.
探讨高血糖对大鼠阴茎组织中硫化氢（H2S）信号通路的影响及其与勃起功能的关系。
将20只8周龄健康雄性斯普拉格-道利（SD）大鼠随机分为A组（4周健康对照组）、B组（4周糖尿病模型组）、C组（6周健康对照组）和D组（6周糖尿病模型组）。B组和D组大鼠腹腔注射链脲佐菌素50 mg/kg诱导糖尿病，A组和C组大鼠注射等体积生理盐水。治疗4周（A组和B组）和6周（C组和D组）后处死动物，通过电刺激测量海绵体内最大压力/平均动脉血压（ICP(max)/MAP），测定血浆和阴茎组织中H2S浓度，采用免疫组织化学和蛋白质印迹法检测阴茎海绵体中胱硫醚-β-合成酶（CBS）和胱硫醚-γ-裂解酶（CSE）的表达。
在5V和7V电刺激盆神经节时，与A组（0.46±0.07和0.68±0.09）和C组（0.43±0.07和0.65±0.16）相比，B组（0.19±0.03和0.29±0.04）和D组（0.14±0.04和0.25±0.04）的ICP(max)/MAP显著降低（P<0.05）。A组和B组（[469.19±126.46] ng/dl对[359.08±60.06] ng/dl，P>0.05）以及C组和D组（[470.44±209.28] ng/dl对[297.01±96.58] ng/dl，P>0.05）之间血清睾酮水平无统计学显著差异。与A组和C组相比，B组和D组的H2S浓度（P<0.05）以及CBS和CSE的表达（P<0.05）显著降低，且D组CBS和CSE的表达比B组下降更显著（P<0.05）。
糖尿病大鼠阴茎海绵体中H2S浓度降低以及CBS和CSE表达减少，提示H2S信号通路可能参与高血糖诱导的勃起功能障碍。
