Crowe R, Burnstock G
Department of Anatomy and Embryology and Centre for Neuroscience, University College London, Gower Street, London WC1E 6BT, U.K.
Int J Dev Neurosci. 1985;3(1):89-101. doi: 10.1016/0736-5748(85)90023-1.
The development of adrenergic (indicated by catecholamine fluorescence), acetylcholine-sterase-positive (possibly cholinergic), non-adrenergic, non-cholinergic (indicated by quinacrine fluorescence) nerves and small intensely fluorescent (SIF) cells in the rabbit urinary bladder was examined in foetal (from 23 days of gestation), newborn and adult animals. Acetylcholinesterase-positive nerve fibres and ganglion cells and quinacrine-positive ganglion cells were both present on day 23 of gestation, while quinacrine-positive varicose nerve fibres were first seen on day 24. At foetal age 26 days, 25-38 ganglia containing quinacrine-positive cells were seen in whole-mount preparations of detrusor muscle of the bladder. Each ganglion contained 30-40 quinacrine-positive cells (diameter 20-40 μm). In contrast, only 5-12 ganglia contained acetylcholinesterase-positive nerve cell bodies at the same foetal age with only 3-20 cells in each ganglion; these figures remained at about the same level from foetal age 23 days to maturity. No catecholamine-containing nerve cell bodies were seen at any foetal age or in the adult. Adrenergic nerve fibres were not detected until day 28 of gestation, although small intensely fluorescent cells were first observed on day 26 of gestation. In the adult bladder there was a reduction of approximately 25-35% in the number of quinacrine-positive nerve cell bodies within the ganglia when compared with the ganglia in 1-day-old bladders and an increase in nerve fibre density of about 50% when compared with bladders of earlier ages. A reduction of approximately 90% in small intensely fluorescent cells and a 2-fold increase of adrenergic nerves was also characteristic of the adult bladder, although no changes were observed in the density of the acetylcholinesterase-positive cell bodies and nerve fibres. It is concluded that catecholamine-containing, acetylcholinesterase-positive and non-adrenergic, non-cholinergic nerves follow very different developmental patterns in the bladder.
在胎儿期（妊娠23天起）、新生期及成年期的兔膀胱中，对肾上腺素能神经（以儿茶酚胺荧光显示）、乙酰胆碱酯酶阳性神经（可能为胆碱能神经）、非肾上腺素能非胆碱能神经（以喹吖因荧光显示）以及小而强荧光细胞（SIF细胞）的发育进行了研究。妊娠23天时，乙酰胆碱酯酶阳性神经纤维和神经节细胞以及喹吖因阳性神经节细胞均已存在，而喹吖因阳性曲张神经纤维于24天时首次出现。在胎儿期26天时，膀胱逼尿肌整装标本中可见25 - 38个含有喹吖因阳性细胞的神经节。每个神经节含有30 - 40个喹吖因阳性细胞（直径20 - 40μm）。相比之下，在相同胎儿期，仅5 - 12个神经节含有乙酰胆碱酯酶阳性神经细胞体，每个神经节仅有3 - 20个细胞；从胎儿期23天到成年，这些数字基本保持在同一水平。在任何胎儿期及成年期均未见含儿茶酚胺的神经细胞体。肾上腺素能神经纤维直到妊娠28天时才被检测到，尽管小而强荧光细胞在妊娠26天时首次被观察到。与1日龄膀胱的神经节相比，成年膀胱神经节内喹吖因阳性神经细胞体数量减少了约25 - 35%；与早期膀胱相比，神经纤维密度增加了约50%。小而强荧光细胞数量减少约90%以及肾上腺素能神经增加2倍也是成年膀胱的特征，尽管乙酰胆碱酯酶阳性细胞体和神经纤维的密度未观察到变化。结论是，含儿茶酚胺神经、乙酰胆碱酯酶阳性神经以及非肾上腺素能非胆碱能神经在膀胱中遵循非常不同的发育模式。
