Ayer-Lelievre C, Dahl D, Björklund H, Seiger A
Spinal Cord Injury Research Laboratory, Harvard Medical School and West Roxbury Veterans Administration Center, Boston, MA 02132, U.S.A.
Department of Histology, Karolinska Institutet, Stockholm, Sweden.
Int J Dev Neurosci. 1985;3(4):385-99. doi: 10.1016/0736-5748(85)90073-5.
Immunoreactivity to neurofilament (NF) antiserum appears early in the development of both the central and peripheral nervous systems of the rat fetus. In 10 somite embryos, positive cell bodies are present in the ventromedial part of anterior rhombencephalic and mesencephalic neural tube. From there the appearance of immunoreactivity spreads cranially to the prosencephalic anlage before closure of the anterior neuropore and caudally following the sequence of neural tube closure. Immunoreactivity increases rapidly in axon bundles of central and peripheral systems, but in immature cell bodies of sensory ganglia the NF material only forms a ring around the nucleus. At 16 days of gestation, some cell bodies are progressively loaded with NF-immunoreactive material as a thick perinuclear network first and then in more excentrically located aggregates. This category of neurons is mainly observed in the distal part of the trigeminal ganglion, in petrous and nodose ganglia and in cervical dorsal root ganglia. In adult ganglia large cell bodies and some small ones present high NF immunoreactivity. In autonomic cell bodies (in superior cervical ganglion and in parasympathetic cranial ganglia) the immunoreactive material only forms a perinuclear ring slowly transformed into a loose perinuciear meshwork at the end of gestation. Intensely reactive nerve fibers are observed in cranial sensory as well as in sympathetic and parasympathetic ganglia and nerves. No positive cell bodies and only a few NF-immunoreactive nerves are observed in the carotid bodies. The NF immunoreactivity is better visualized on sections of fresh frozen material, treated with acetone, than in fixed specimens. These results are compared to previous observations reported for other species and for developing dorsal root ganglia. This immunostaining may be used to detect differentiation of peripheral sensory and autonomic neurons under experimental conditions. The uneven distribution of NF immunoreactivity in sensory neurons from stage 16 days of gestation as specific for precise subpopulations of neurons is discussed.
对神经丝（NF）抗血清的免疫反应性在大鼠胎儿中枢和外周神经系统发育早期就已出现。在10体节胚胎中，前菱脑和中脑神经管腹内侧部分存在阳性细胞体。从那里开始，免疫反应性先向头侧扩散至前脑原基，此时前神经孔尚未闭合，然后随着神经管闭合的顺序向尾侧扩散。中枢和外周系统的轴突束中免疫反应性迅速增加，但在感觉神经节的未成熟细胞体中，NF物质仅在细胞核周围形成一个环。在妊娠16天时，一些细胞体逐渐充满NF免疫反应性物质，首先形成浓密的核周网络，然后形成更偏心分布的聚集体。这类神经元主要见于三叉神经节远端、岩神经节和结状神经节以及颈背根神经节。在成年神经节中，大细胞体和一些小细胞体呈现高NF免疫反应性。在自主神经细胞体（颈上神经节和副交感神经颅神经节）中，免疫反应性物质仅形成一个核周环，在妊娠末期缓慢转变为松散的核周网状结构。在颅感觉神经节以及交感和副交感神经节和神经中观察到强反应性神经纤维。在颈动脉体中未观察到阳性细胞体，仅观察到少数NF免疫反应性神经。与用丙酮处理的新鲜冷冻材料切片相比，在固定标本中NF免疫反应性更易观察到。将这些结果与先前报道的其他物种和发育中的背根神经节的观察结果进行了比较。这种免疫染色可用于在实验条件下检测外周感觉和自主神经元的分化。讨论了妊娠16天起感觉神经元中NF免疫反应性的不均匀分布，这对神经元的精确亚群具有特异性。
