Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal.
Instituto de Ciência e Engenharia de Materiais e Superficies (ICEMS), Instituto Superior Técnico, Universidade Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
Bioresour Technol. 2014 Jul;164:362-70. doi: 10.1016/j.biortech.2014.04.108. Epub 2014 May 9.
The immobilization of naringinase in PVA lens-shaped particles, a cheap and biocompatible hydrogel was shown to provide an effective biocatalyst for naringin hydrolysis, an appealing reaction in the food and pharmaceutical industries. The present work addresses the operational stability and scale-up of the bioconversion system, in various types of reactors, namely shaken microtiter plates (volume ⩽ 2 mL), batch stirred tank reactors (volume <400 mL) and a packed-bed reactor (PBR, 6.8 mL). Consecutive batch runs were performed with the shaken/stirred vessels, with reproducible and encouraging results, related to operational stability. The PBR was used to establish the feasibility for continuous operation, running continuously for 54 days at 45°C. The biocatalyst activity remained constant for 40 days of continuous operation. The averaged specific productivity was 9.07 mmol h(-1) g enzyme(-1) and the half-life of 48 days.
将柚皮苷酶固定在 PVA 透镜状颗粒中，这种廉价且具有生物相容性的水凝胶被证明是一种有效的酶，可用于水解柚皮苷，这是食品和制药行业中很有吸引力的反应。本工作研究了在不同类型的反应器中，即摇微滴定板（体积 ⩽ 2 毫升）、分批搅拌釜反应器（体积 <400 毫升）和填充床反应器（PBR，6.8 毫升）中，生物转化系统的操作稳定性和放大问题。用摇床/搅拌釜进行了连续分批运行，结果具有可重复性和令人鼓舞的操作稳定性。使用 PBR 来确定连续操作的可行性，在 45°C 下连续运行 54 天。生物催化剂的活性在连续运行 40 天后保持不变。平均比生产能力为 9.07 mmol h(-1) g 酶(-1)，半衰期为 48 天。
