Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade Lisboa, Av. Gama Pinto, 1649-003 Lisboa, Portugal.
Instituto de Ciência e Engenharia de Materiais e Superfícies, Departamento de Engenharia de Materiais, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
Bioresour Technol. 2016 Aug;213:208-215. doi: 10.1016/j.biortech.2016.03.058. Epub 2016 Mar 14.
Polyvinyl alcohol (PVA) electrospun nanofibers were produced using an electrospinning technique. Key parameters (e.g. collectors, distance from needle tip to collector, among others) that influence the structure and morphology of fibers were optimized. The naringinase entrapped in PVA nanofibers retained over 100% of its initial activity after 212h of operation, at 25°C. Chemical crosslinking with several boronic acids further increased the hydrolysis temperature (up to 85°C) and yielded nanofibers with thermal stability up to 121°C. A mini packed bed reactor (PBR) developed to establish the feasibility for continuous enzymatic operation, ran for 16days at 45°C. Highest naringenin biosynthesis was attained at a flow rate of 10mLh(-1). Highest volumetric (78molL(-1)h(-1)) and specific (26molh(-1)genzyme(-1)) productivities were attained at 30mLh(-1). The activity of NGase in electrospun nanofibers remained constant for almost 16days of operation at 10mLh(-1).
聚乙烯醇（PVA）纳米纤维是采用静电纺丝技术制备的。优化了影响纤维结构和形态的关键参数（例如收集器、针尖到收集器的距离等）。在 25°C 下，固定在 PVA 纳米纤维中的柚皮苷酶在 212 小时的操作后保留了超过 100%的初始活性。用几种硼酸进行化学交联进一步提高了水解温度（高达 85°C），并得到了热稳定性高达 121°C 的纳米纤维。为了建立连续酶法操作的可行性而开发的微型填充床反应器（PBR）在 45°C 下运行了 16 天。在流速为 10mLh(-1)时，达到了最高的柚皮素生物合成。在 30mLh(-1)时，达到了最高的比（26molh(-1)genzyme(-1)）和总体积（78molL(-1)h(-1)）产率。在 10mLh(-1)的流速下，NGase 在静电纺丝纳米纤维中的活性在近 16 天的运行中保持不变。
