基于 PCR 的中间普雷沃菌鉴定新型引物的开发和评估。
Development and evaluation of new primers for PCR-based identification of Prevotella intermedia.
机构信息
Department of Periodontology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, 639 Zhi Zao Ju Road, Shanghai 210011, China.
Shanghai Research Institute of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 210011, China.
出版信息
Anaerobe. 2014 Aug;28:126-9. doi: 10.1016/j.anaerobe.2014.05.005. Epub 2014 May 27.
The aim of this study was to develop new Prevotella intermedia-specific PCR primers based on the 16S rRNA. The new primer set, Pi-192 and Pi-468, increased the accuracy of PCR-based P. intermedia identification and could be useful in the detection of P. intermedia as well as epidemiological studies on periodontal disease.
本研究旨在基于 16S rRNA 开发新的中间普氏菌特异性 PCR 引物。新的引物对 Pi-192 和 Pi-468 提高了基于 PCR 的中间普氏菌鉴定的准确性，可用于中间普氏菌的检测以及牙周病的流行病学研究。
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