Xi Lei, Song Chaolong, Jiang Huabei
Opt Lett. 2014 Jun 1;39(11):3328-31. doi: 10.1364/OL.39.003328.
Photoacoustic microscopy (PAM) has remained one of the fastest developing biomedical imaging modalities in the past decade. The confocal strategy of optical illumination and acoustic detection is a way to boost the sensitivity of PAM. To achieve confocal PAM, current PAM systems utilize separate acoustic and optical converging devices, making the systems bulky and complicated. In this Letter, we demonstrate the use of a single-liquid lens to successfully achieve acoustic and optical confocal configuration for optical-resolution PAM (ORPAM). Using the lens with a numerical aperture of 0.43, we show that the resolution of the ORPAM system is 4.8 μm with a significantly improved sensitivity of acoustic detection. We also apply this compact ORPAM system to in vivo imaging of the vasculature of a rat ear.
在过去十年中，光声显微镜（PAM）一直是发展最快的生物医学成像模式之一。光学照明和声学检测的共聚焦策略是提高PAM灵敏度的一种方法。为了实现共聚焦PAM，目前的PAM系统使用单独的声学和光学会聚装置，这使得系统体积庞大且复杂。在本信函中，我们展示了使用单个液体透镜成功实现光学分辨率PAM（ORPAM）的声学和光学共聚焦配置。使用数值孔径为0.43的透镜，我们表明ORPAM系统的分辨率为4.8μm，声学检测灵敏度显著提高。我们还将这种紧凑的ORPAM系统应用于大鼠耳部血管的体内成像。
