Jiang Bowen, Yang Xiaoquan, Liu Yanyan, Deng Yong, Luo Qingming
Opt Lett. 2014 Jul 1;39(13):3939-41. doi: 10.1364/OL.39.003939.
A multiscale photoacoustic microscope with continuously tunable lateral resolution is developed. The tunable resolution is achieved by using an electrical varifocal lens and an optical fiber bundle. The varifocal lens is used to generate a size tunable focused laser spot on the tip of the fiber bundle. Laser beams emerging from the other end of the fiber bundle are imaged into the object as the excitation light spot for acoustic generation. The verified lateral resolution of the system can be tuned from ~1 μm to more than 44.8 μm, which span from optical resolution to acoustic resolution. Additionally, a mouse ear was imaged in vivo using three different resolutions to demonstrate the feasibility of the multiscale imaging capability of our system.
开发了一种具有连续可调横向分辨率的多尺度光声显微镜。通过使用电变焦距透镜和光纤束实现可调分辨率。变焦距透镜用于在光纤束的尖端产生尺寸可调的聚焦激光光斑。从光纤束另一端出射的激光束作为用于产生声波的激发光斑成像到物体中。该系统经证实的横向分辨率可从约1μm调至超过44.8μm，跨越了从光学分辨率到声学分辨率的范围。此外，使用三种不同分辨率对小鼠耳朵进行了体内成像，以证明我们系统多尺度成像能力的可行性。
