人房水中 MMP-2、MMP-3、TIMP-1、TIMP-2 和 TIMP-3 蛋白水平：与眼轴长度的关系。

MMP-2, MMP-3, TIMP-1, TIMP-2, and TIMP-3 protein levels in human aqueous humor: relationship with axial length.

机构信息

Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, People's Republic of China.

Departments of Ophthalmology and Pathology, New York Eye and Ear Infirmary, New York, New York, United States.

出版信息

Invest Ophthalmol Vis Sci. 2014 May 29;55(6):3922-8. doi: 10.1167/iovs.14-13983.

DOI:10.1167/iovs.14-13983

PMID:24876280

Abstract

PURPOSE

We measured the aqueous humor levels of matrix metalloproteinases (MMP) MMP-1, MMP-2, MMP-3, and tissue inhibitors of matrix metalloproteinases (TIMP) TIMP-1, TIMP-2, and TIMP-3 in patients with myopia or cataract, and investigated the relationship between their levels and axial length (AL).

METHODS

We measured MMP/TIMPs levels with the Luminex xMAP Technology by using commercially available Milliplex xMAP Kits. A total of 65 aqueous humor samples was collected from patients with myopia or cataract during cataract or clear lens extraction surgery. According to the AL, the samples were divided into three groups: group A, AL ≤ 24 mm; group B, AL 24 to 26 mm; and group C, AL ≥ 26 mm.

RESULTS

Levels of MMP-2, MMP-3, TIMP-1, TIMP-2, and TIMP-3 could be detected in the aqueous humor. The levels of MMP-2, TIMP-1, TIMP-2, and TIMP-3 were positively correlated with AL. The differences of the levels of these MMPs/TIMPs among the three groups were statistically significant. The MMP-3 levels were not correlated with AL and there was no significant difference in MMP-3 levels among these three groups. Levels of MMP-1 could not be detected in the aqueous humor samples.

CONCLUSIONS

Elevated levels of aqueous MMP-2, TIMP-1, TIMP-2, and TIMP-3 were found in the eyes with elongated axis.

摘要

目的

我们测量了近视或白内障患者房水中基质金属蛋白酶（MMP）MMP-1、MMP-2、MMP-3 以及基质金属蛋白酶组织抑制剂（TIMP）TIMP-1、TIMP-2 和 TIMP-3 的水平，并探讨了它们的水平与眼轴（AL）之间的关系。

方法

我们使用商业可得的 Milliplex xMAP 试剂盒，通过 Luminex xMAP 技术测量 MMP/TIMP 水平。共收集了 65 例白内障或透明晶状体摘除术中近视或白内障患者的房水样本。根据 AL 将样本分为三组：A 组，AL≤24mm；B 组，AL 为 24 至 26mm；C 组，AL≥26mm。

结果

可以在房水中检测到 MMP-2、MMP-3、TIMP-1、TIMP-2 和 TIMP-3 的水平。MMP-2、TIMP-1、TIMP-2 和 TIMP-3 的水平与 AL 呈正相关。这些 MMP/TIMP 水平在三组之间的差异具有统计学意义。MMP-3 水平与 AL 无关，三组之间 MMP-3 水平无显著差异。MMP-1 水平在房水样本中无法检测到。