Wahyuningsih Eka, Wigid Dimas, Dewi Astrid, Moehariadi Hariwati, Sujuti Hidayat, Anandita Nanda
Department of Ophthalmology, Faculty of Medicine, Universitas Brawijaya, Dr. Saiful Anwar General Hospital, Malang 65111, Indonesia.
Biomedicines. 2022 Oct 17;10(10):2600. doi: 10.3390/biomedicines10102600.
Citicoline, presumed to be involved in the dopaminergic pathway, might play a role as a candidate agent in controlling myopia. However, its study with respect to myopia is limited. The aim of this study is to demonstrate the effect of citicoline on the expression of MMP-2, TGF-β1, and Ki-67, and on the thickness of scleral tissue of a rat myopia model. Immunohistochemistry was performed to evaluate the expression of MMP-2, TGF-β1, and Ki-67 as the markers for fibroblast proliferation. Hematoxylin and eosin staining were used to evaluate scleral thickness. An electronic digital caliper was used to evaluate the axial length. The treatment group administered with 200 mg/kg BW/day had the lowest mean MMP-2 expression, axial elongation, and fibroblast proliferation, but it had the highest mean scleral thickness. The treatment group administered with 300 mg/kg BW/day had the highest mean TGF-β1 expression. Citicoline is able to decrease MMP-2 expression and fibroblast proliferation and increase TGF-β1 expression and scleral tissue thickness significantly in the scleral tissue of rat models for myopia.
胞磷胆碱被认为参与多巴胺能通路，可能作为控制近视的候选药物发挥作用。然而，关于其对近视的研究有限。本研究的目的是证明胞磷胆碱对大鼠近视模型中基质金属蛋白酶-2（MMP-2）、转化生长因子-β1（TGF-β1）和Ki-67表达以及巩膜组织厚度的影响。采用免疫组织化学法评估MMP-2、TGF-β1和Ki-67的表达，作为成纤维细胞增殖的标志物。苏木精-伊红染色用于评估巩膜厚度。使用电子数字卡尺评估眼轴长度。给予200mg/kg体重/天的治疗组平均MMP-2表达、眼轴伸长和成纤维细胞增殖最低，但平均巩膜厚度最高。给予300mg/kg体重/天的治疗组平均TGF-β1表达最高。在大鼠近视模型的巩膜组织中，胞磷胆碱能够显著降低MMP-2表达和成纤维细胞增殖，并增加TGF-β1表达和巩膜组织厚度。
