Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, Texas, United States.
Invest Ophthalmol Vis Sci. 2014 May 29;55(7):4229-37. doi: 10.1167/iovs.14-14513.
To evaluate the effects of a chemically preserved multipurpose contact lens care solution (MPS) on the corneal epithelial surface and Pseudomonas aeruginosa (PA) internalization in the rabbit corneal epithelium.
Rabbits were fit in one eye with a silicone hydrogel lens (balafilcon A) soaked overnight in a borate-buffered MPS (BioTrue). The contralateral eye was fit with a lens removed directly from the blister pack containing borate-buffered saline (control). Lenses were worn for 2 hours. Upon lens removal, corneas were challenged ex vivo with invasive PA strain 6487 and assessed for PA internalization. Ultrastructural changes were assessed using scanning electron (SEM) and transmission electron microscopy (TEM).
Scanning electron microscopy showed frank loss of surface epithelium in MPS-exposed eyes, while control eyes exhibited occasional loss of surface membranes but retention of intact junctional borders. Transmission electron microscopy data supported and extended SEM findings, demonstrating the presence of epithelial edema in MPS-treated eyes. There was a 12-fold increase in PA uptake into the corneal epithelium following wear of the MPS-treated lens compared to control (P = 0.008).
These data demonstrate that corneal exposure to MPS during lens wear damages the surface epithelium and are consistent with our previous clinical data showing an increase in bacterial binding to exfoliated epithelial cells following MPS use with resultant increased risk for lens-mediated infection. These findings also demonstrate that the PA invasion assay may provide a highly sensitive quantitative metric for assessing the physiological impact of lens-solution biocompatibility on the corneal epithelium.
评估一种化学保存的多用途接触镜护理液（MPS）对兔角膜上皮表面的影响，以及对铜绿假单胞菌（PA）内化的影响。
将兔子的一只眼睛用硅水凝胶镜片（balafilcon A）适配，镜片在硼酸盐缓冲的 MPS（BioTrue）中浸泡过夜。对侧眼睛用从含有硼酸盐缓冲盐水的泡罩包装中直接取出的镜片适配（对照）。镜片佩戴 2 小时。镜片取出后，将角膜在体外用侵袭性 PA 株 6487 进行挑战，并评估 PA 内化。使用扫描电子显微镜（SEM）和透射电子显微镜（TEM）评估超微结构变化。
扫描电子显微镜显示，在 MPS 暴露的眼睛中，表面上皮明显丧失，而对照眼睛仅偶尔丧失表面膜，但保留完整的连接边界。透射电子显微镜数据支持并扩展了 SEM 发现，表明 MPS 治疗的眼睛存在上皮水肿。与对照相比，佩戴 MPS 处理镜片后，PA 摄取到角膜上皮的量增加了 12 倍（P = 0.008）。
这些数据表明，在佩戴镜片期间，角膜暴露于 MPS 会损伤表面上皮，这与我们之前的临床数据一致，表明在使用 MPS 后，细菌与脱落的上皮细胞结合增加，从而导致镜片介导的感染风险增加。这些发现还表明，PA 入侵测定法可能为评估镜片溶液生物相容性对角膜上皮的生理影响提供一种高度敏感的定量指标。
