Gorbet M B, Tanti N C, Crockett B, Mansour L, Jones L
Department of Systems Design Engineering, University of Waterloo, Waterloo, Ontario, Canada.
Mol Vis. 2011;17:3458-67. Epub 2011 Dec 28.
Multipurpose solutions (MPS) are used daily to clean and disinfect silicone hydrogel (SiHy) contact lenses. This in vitro study was undertaken to identify the potential for interaction between MPS, SiHy surface treatments, and lens materials, which may lead to changes in the response of human corneal epithelial cells (HCEC) to MPS-soaked lenses.
The MPS tested were renu fresh (formerly known as ReNu MultiPlus; ReNu), OptiFree Express (OFX), OptiFree RepleniSH, SoloCare Aqua, and Complete Moisture Plus. The SiHy materials evaluated were lotrafilcon A, lotrafilcon B, comfilcon A, galyfilcon A, and balafilcon A (BA). MPS-soaked lenses were placed on top of adherent HCEC. The effect of MPS dilutions (0.1 to 10% final concentration in medium) was also characterized. Cell viability, adhesion phenotype and caspase activation were studied after 24-h cell exposure. OFX released from lenses was determined using UV absorbance.
A significant reduction in viability (between 30 to 50%) was observed with cells exposed to lenses soaked in ReNu and OFX. A significant downregulation of α(3) and β(1) integrins, with integrin expression ranging from 60% to 75% of control (cells with no lens), was also observed with OFX and ReNu-soaked lenses. With the exception of BA, all other lenses soaked in OFX resulted in significant caspase activation, whereby over 18% of cells stained positive for caspases. Minimal caspase activation was observed in cells exposed to ReNu and Solo soaked lenses. For both OFX and ReNu, exposing cells to at least a 5% dilution had a significant effect on viability and integrin expression. While Complete and Solo did not lead to reduction in viability, cells exposed to a 10% dilution showed reduced integrin expression down to less than 70% of control value. Comparing cell response to diluted MPS solutions and various MPS-soaked lenses showed that it is not possible to reliably use cell response to MPS dilution alone to assess MPS biocompatibility.
Our results demonstrate that the reaction of HCEC to MPS are affected by the type of lenses the MPS is released from and may potentially be influenced by the surface treatment (or lack of it) of SiHy materials.
多功能护理液（MPS）每日用于清洁和消毒硅水凝胶（SiHy）隐形眼镜。本体外研究旨在确定MPS、SiHy表面处理和镜片材料之间相互作用的可能性，这可能导致人角膜上皮细胞（HCEC）对浸泡在MPS中的镜片的反应发生变化。
测试的MPS有润明清新护理液（原名润明多效护理液；润明）、傲滴恒润护理液（OFX）、傲滴全护理液、乐敦清护理液和全效保湿护理液。评估的SiHy材料有lotrafilcon A、lotrafilcon B、comfilcon A、galyfilcon A和balafilcon A（BA）。将浸泡在MPS中的镜片放置在贴壁的HCEC上。还对MPS稀释液（培养基中终浓度为0.1%至10%）的效果进行了表征。在细胞暴露24小时后研究细胞活力、黏附表型和半胱天冬酶激活情况。使用紫外吸光度测定从镜片中释放的OFX。
观察到暴露于浸泡在润明和OFX中的镜片的细胞活力显著降低（30%至50%）。浸泡在OFX和润明中的镜片还观察到α(3)和β(1)整合素显著下调，整合素表达范围为对照（无镜片的细胞）的60%至75%。除BA外，浸泡在OFX中的所有其他镜片均导致显著的半胱天冬酶激活，超过18%的细胞半胱天冬酶染色呈阳性。在暴露于浸泡在润明和乐敦清中的镜片的细胞中观察到最小程度的半胱天冬酶激活。对于OFX和润明，将细胞暴露于至少5%的稀释液对活力和整合素表达有显著影响。虽然全效保湿护理液和乐敦清护理液不会导致活力降低，但暴露于10%稀释液的细胞显示整合素表达降低至对照值的70%以下。比较细胞对稀释的MPS溶液和各种浸泡在MPS中的镜片的反应表明，仅根据细胞对MPS稀释液的反应来可靠评估MPS生物相容性是不可能的。
我们的结果表明，HCEC对MPS的反应受MPS所释放的镜片类型影响，并且可能潜在地受SiHy材料的表面处理（或未处理）影响。
