基于微生物的方法检测牛奶中β-内酰胺类抗生素的特异性。
Microbial based assay for specific detection of β-lactam group of antibiotics in milk.
机构信息
Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001 Haryana India.
The ICFAI University, Tripura, Kamalghat Sadar, Agarthala, 799210 West Tripura, India.
出版信息
J Food Sci Technol. 2014 Jun;51(6):1161-6. doi: 10.1007/s13197-011-0609-4. Epub 2011 Dec 24.
The spore forming Bacillus cereus (66) was screened for the induction of β-lactamase in presence of an inducer using iodometric assay. A significant induction in marker enzyme was observed in B. cereus 66 at maximum residual limit (MRL) of penicillin, ampicillin, cloxacillin, amoxicillin, cefalexin, and cephazolin belonging to β-lactam group of antibiotics. A microbial based assay, where enzyme induction was optimized at pH 7.0, temperature 30°C, and whey powder (0.25%) after 4 h of incubation. The spore based assay was tested with milk samples spiked with 6 different β-lactam antibiotics. The results were 100 and 83.33% in correlation with microbial receptor and inhibition based assay, respectively. Overall, spore based assay can be a useful and cost effective tool for the specific detection of β-lactam group of antibiotics in milk.
从芽孢形成的蜡样芽胞杆菌（66）中筛选出在诱导物存在下使用碘量法检测β-内酰胺酶的诱导物。在青霉素、氨苄西林、氯唑西林、阿莫西林、头孢氨苄和头孢唑林等β-内酰胺类抗生素的最大残留限量（MRL）下，观察到蜡样芽胞杆菌 66 中标记酶有显著诱导。在 pH 值为 7.0、温度为 30°C 和乳清粉（0.25%）孵育 4 小时后，优化了基于微生物的酶诱导测定法。基于孢子的测定法用于检测添加了 6 种不同β-内酰胺抗生素的牛奶样品。与微生物受体和抑制基础测定法的相关性分别为 100%和 83.33%。总的来说，基于孢子的测定法可以成为一种有用且具有成本效益的工具，用于在牛奶中特异性检测β-内酰胺类抗生素。
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