Saris N E, Somerharju P
Department of Medical Chemistry, University of Helsinki, Finland.
Acta Chem Scand (Cph). 1989 Jan;43(1):82-5. doi: 10.3891/acta.chem.scand.43-0082.
A sensitive phospholipase A assay suitable for organelle activities is described. Activation of the enzyme produces an increase in membrane lysophospholipids. The amino groups of extracted lipids were derivatized with 4-fluoro-7-nitrobenzo-2-oxa-1,3-diazole and the amounts of phosphatidylethanolamine and its lysoform were quantitated using HPLC equipped with a SiO2 column and a fluorescence detector. The procedure was tested with porcine enzyme acting on liposomes and mitochondria, and with endogenous mitochondrial enzyme.
本文描述了一种适用于细胞器活性检测的灵敏磷脂酶A测定方法。该酶的激活会导致膜溶血磷脂增加。提取的脂质氨基用4-氟-7-硝基苯并-2-恶唑-1,3-二氮杂茂进行衍生化，使用配备二氧化硅柱和荧光检测器的高效液相色谱法定量磷脂酰乙醇胺及其溶血形式的含量。该方法已用于检测作用于脂质体和线粒体的猪源酶以及内源性线粒体酶。
