Sampaio João P A, Cavalcante José R, Furtado Francisco N N, Lima-Júnior Roberto C P, Ribeiro Ronaldo A, Almeida Paulo R C
Department of Pathology and Forensic Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.
Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.
J Pharmacol Toxicol Methods. 2014 Jul-Aug;70(1):70-2. doi: 10.1016/j.vascn.2014.05.005. Epub 2014 May 28.
Tissue microarray (TMA) was first designed to enable more efficient immunohistochemical screening of antibodies and tissues. However, due to the high cost of commercial TMA builder instrument, such method is not affordable for many pathology laboratories. Then, methodological adaptations have been proposed in order to reduce TMA-associated cost.
A manual leather puncher with an inner diameter of 2mm was used to collect a tissue sample from the donor paraffin block. The conventional TMA method was adopted as a control group.
Empty paraffin recipient blocks were prepared and a standard 2-mm crochet needle was used to create 24 equidistant holes in the recipient block. Tissue cores obtained from the donor blocks were transferred to the holes in the recipient blocks and routine histopathological techniques were then performed.
In this study we proposed a new approach to produce TMA recipient blocks as an alternative to the conventional TMA.
组织微阵列（TMA）最初设计用于更高效地对抗体和组织进行免疫组织化学筛选。然而，由于商业TMA构建仪器成本高昂，许多病理实验室难以承受这种方法。因此，人们提出了方法上的改进以降低与TMA相关的成本。
使用内径为2mm的手动皮革打孔器从供体石蜡块中采集组织样本。采用传统TMA方法作为对照组。
制备了空白石蜡受体块，并使用标准的2mm钩针在受体块上打24个等距孔。从供体块获得的组织芯转移到受体块的孔中，然后进行常规组织病理学技术操作。
在本研究中，我们提出了一种制备TMA受体块的新方法，作为传统TMA的替代方法。
