Gologan Daniela, Ștefan Alina Elena, Militaru Manuella, Sanda Andreea Cristina, Arjan Suzana, Mușat Sorin, Leavitt Matthew Okerlund, Stan Raluca
Department of Organic Chemistry, Doctoral School, Faculty of Chemical Engineering and Biotechnologies, Politehnica University, 011061 Bucharest, Romania.
Department of Research and Development, Themis Pathology SRL, 077190 Bucharest, Romania.
Vet Sci. 2023 Apr 6;10(4):280. doi: 10.3390/vetsci10040280.
In the context of cost increases of both labor and consumables, cheaper and faster histopathology methods are needed. We implemented in our research laboratory the use of tissue microarrays (TMA) for the parallel processing and analysis of tissue samples. In this study, we used seven pre-processed, paraffinated biomimetic sectionable support matrices serving as "recipient" paraffin blocks to embed a total of 196 tissue cores from formalin-fixed paraffin-embedded tissue samples (serving as "donor" paraffin blocks) from seven different rabbit organs. These tissue samples were obtained using four different processing protocols: two 6 h protocols with xylene as the transition solvent, and two using butanol instead (one 10 h in duration and the other 72 h long). While the samples from protocols 1 and 2 (with xylene) quite regularly generated peeling of some of the cores from the slides (most likely because of substandard paraffin infiltration), butanol processing performed flawlessly for both processing protocols. Our proposed technique of using TMAs in the research laboratory brings with it a significant reduction in time and consumable costs (up to 77 and 64%, respectively), but also new challenges for all the upstream processes.
在劳动力和耗材成本都增加的背景下，需要更便宜、更快的组织病理学方法。我们在研究实验室中采用了组织微阵列（TMA）来并行处理和分析组织样本。在本研究中，我们使用了七个预处理的、石蜡包埋的仿生可切片支持基质作为“受体”石蜡块，以嵌入来自七个不同兔器官的福尔马林固定石蜡包埋组织样本（作为“供体”石蜡块）中的总共196个组织芯。这些组织样本使用四种不同的处理方案获得：两种以二甲苯为过渡溶剂的6小时方案，以及另外两种使用丁醇替代的方案（一种持续10小时，另一种长达72小时）。虽然方案1和2（使用二甲苯）的样本相当频繁地出现一些组织芯从载玻片上脱落的情况（很可能是由于石蜡浸润不达标），但丁醇处理在两种处理方案中都表现完美。我们在研究实验室中使用TMA的技术显著降低了时间和耗材成本（分别高达77%和64%），但也给所有上游流程带来了新的挑战。
