Hu Baolan, He Zhanfei, Geng Sha, Cai Chen, Lou Liping, Zheng Ping, Xu Xinhua
Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China.
Appl Microbiol Biotechnol. 2014 Sep;98(18):7983-91. doi: 10.1007/s00253-014-5835-z. Epub 2014 Jun 1.
Nitrite-dependent anaerobic methane oxidation (n-damo) is mediated by bacteria that anaerobically oxidize methane coupled with nitrite reduction and is a potential bioprocess for wastewater treatment. In this work, the effect of reactor configuration on n-damo bacterial cultivation was investigated. A magnetically stirred gas lift reactor (MSGLR), a sequencing batch reactor (SBR), and a continuously stirred tank reactor (CSTR) were selected to cultivate the bacteria. Microbial community was monitored by using quantitative PCR, 16S rRNA gene sequencing, pmoA gene sequencing, and fluorescence in situ hybridization (FISH). The effects of substrate inhibition, methane mass transfer, and biomass washout in the three reactors were focused on. The results indicated that the MSGLR had the best performance among the three reactor systems, with the highest total and specific n-damo activities. Its maximum volumetric nitrogen removal rate was up to 76.9 mg N L(-1) day(-1), which was higher than previously reported values (5.1-37.8 mg N L(-1) d(-1)).
亚硝酸盐依赖型厌氧甲烷氧化（n-damo）由细菌介导，这些细菌厌氧氧化甲烷并耦合亚硝酸盐还原，是一种潜在的废水处理生物过程。在本研究中，考察了反应器构型对n-damo细菌培养的影响。选择了磁力搅拌气升式反应器（MSGLR）、序批式反应器（SBR）和连续搅拌槽式反应器（CSTR）来培养细菌。通过定量PCR、16S rRNA基因测序、pmoA基因测序和荧光原位杂交（FISH）监测微生物群落。重点研究了三种反应器中底物抑制、甲烷传质和生物量冲刷的影响。结果表明，在三种反应器系统中，MSGLR性能最佳，具有最高的总n-damo活性和比n-damo活性。其最大容积氮去除率高达76.9 mg N L(-1) d(-1)，高于先前报道的值（5.1-37.8 mg N L(-1) d(-1)）。
