Thoen C O, Moore L A
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011.
J Vet Diagn Invest. 1989 Jul;1(3):223-6. doi: 10.1177/104063878900100305.
A 6-year study was conducted in 4 dairy herds in Iowa in which Johne's disease was diagnosed previously. Fecal specimens were collected at 6-month intervals from animals 2 years of age and over for mycobacteriologic examination. Serum samples were obtained at 3-month intervals and tested by enzyme-linked immunosorbent assay (ELISA). The antigen used in the ELISA was a potassium chloride extract of a field strain of Mycobacterium paratuberculosis. The ELISA reactions were observed in 87% of the cows from which M. paratuberculosis was isolated. Dairy producers that participated in the Johne's control program reported reduced economic losses. Increased income was attributed to improved milk production, increased value of vaccinated animals sold as replacements to other dairy herds in which Johne's disease had been diagnosed, and the increased market value of slaughter animals removed from the herd.
在爱荷华州先前已诊断出患有副结核病的4个奶牛场进行了一项为期6年的研究。每隔6个月从2岁及以上的动物采集粪便样本进行分枝杆菌学检查。每隔3个月采集血清样本并通过酶联免疫吸附测定（ELISA）进行检测。ELISA中使用的抗原是副结核分枝杆菌田间菌株的氯化钾提取物。在分离出副结核分枝杆菌的奶牛中，87%观察到ELISA反应呈阳性。参与副结核病控制计划的奶农报告称经济损失减少。收入增加归因于牛奶产量提高、作为替代牛出售给其他已诊断出患有副结核病的奶牛场的接种动物价值增加，以及从牛群中淘汰的屠宰动物市场价值增加。
