水稻钙依赖蛋白激酶OsCPK9正向调控干旱胁迫耐受性和小穗育性。
A rice calcium-dependent protein kinase OsCPK9 positively regulates drought stress tolerance and spikelet fertility.
作者信息
Wei Shuya, Hu Wei, Deng Xiaomin, Zhang Yingying, Liu Xiaodong, Zhao Xudong, Luo Qingchen, Jin Zhengyi, Li Yin, Zhou Shiyi, Sun Tao, Wang Lianzhe, Yang Guangxiao, He Guangyuan
机构信息
The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China.
出版信息
BMC Plant Biol. 2014 May 17;14:133. doi: 10.1186/1471-2229-14-133.
BACKGROUND
In plants, calcium-dependent protein kinases (CDPKs) are involved in tolerance to abiotic stresses and in plant seed development. However, the functions of only a few rice CDPKs have been clarified. At present, it is unclear whether CDPKs also play a role in regulating spikelet fertility.
RESULTS
We cloned and characterized the rice CDPK gene, OsCPK9. OsCPK9 transcription was induced by abscisic acid (ABA), PEG6000, and NaCl treatments. The results of OsCPK9 overexpression (OsCPK9-OX) and OsCPK9 RNA interference (OsCPK9-RNAi) analyses revealed that OsCPK9 plays a positive role in drought stress tolerance and spikelet fertility. Physiological analyses revealed that OsCPK9 improves drought stress tolerance by enhancing stomatal closure and by improving the osmotic adjustment ability of the plant. It also improves pollen viability, thereby increasing spikelet fertility. In OsCPK9-OX plants, shoot and root elongation showed enhanced sensitivity to ABA, compared with that of wild-type. Overexpression and RNA interference of OsCPK9 affected the transcript levels of ABA- and stress-responsive genes.
CONCLUSIONS
Our results demonstrated that OsCPK9 is a positive regulator of abiotic stress tolerance, spikelet fertility, and ABA sensitivity.
背景
在植物中，钙依赖蛋白激酶（CDPKs）参与非生物胁迫耐受性及植物种子发育过程。然而，仅有少数水稻CDPKs的功能得以阐明。目前，尚不清楚CDPKs是否在调控小穗育性方面发挥作用。
结果
我们克隆并鉴定了水稻CDPK基因OsCPK9。OsCPK9的转录受脱落酸（ABA）、聚乙二醇6000（PEG6000）和氯化钠（NaCl）处理诱导。OsCPK9过表达（OsCPK9-OX）和OsCPK9 RNA干扰（OsCPK9-RNAi）分析结果表明，OsCPK9在干旱胁迫耐受性和小穗育性方面发挥积极作用。生理分析显示，OsCPK9通过增强气孔关闭和提高植物渗透调节能力来提高干旱胁迫耐受性。它还能提高花粉活力，从而增加小穗育性。与野生型相比，在OsCPK9-OX植株中，地上部和根部伸长对ABA表现出增强的敏感性。OsCPK9的过表达和RNA干扰影响了ABA及胁迫响应基因的转录水平。
结论
我们的结果表明，OsCPK9是一种非生物胁迫耐受性、小穗育性和ABA敏感性的正向调节因子。
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