评估香精油对杜克雷嗜血杆菌的抗菌潜力。
Assessing the antibiotic potential of essential oils against Haemophilus ducreyi.
作者信息
Lindeman Zachary, Waggoner Molly, Batdorff Audra, Humphreys Tricia L
机构信息
Allegheny College Department of Biology, 520 N, Main St,, Meadville, PA 16335, USA.
出版信息
BMC Complement Altern Med. 2014 May 27;14:172. doi: 10.1186/1472-6882-14-172.
BACKGROUND
Haemophilus ducreyi is the bacterium responsible for the genital ulcer disease chancroid, a cofactor for the transmission of HIV, and it is resistant to many antibiotics. With the goal of exploring possible alternative treatments, we tested essential oils (EOs) for their efficacy as antimicrobial agents against H. ducreyi.
METHODS
We determine the minimum inhibitory concentration (MIC) of Cinnamomum verum (cinnamon), Eugenia caryophyllus (clove) and Thymus satureioides (thyme) oil against 9 strains of H. ducreyi using the agar dilution method. We also determined the minimum lethal concentration for each oil by subculturing from the MIC plates onto fresh agar without essential oil. For both tests, we used a 2-way ANOVA to evaluate whether antibiotic-resistant strains had a different sensitivity to the oils relative to non-resistant strains.
RESULTS
All 3 oils demonstrated excellent activity against H. ducreyi, with MICs of 0.05 to 0.52 mg/mL and MLCs of 0.1-0.5 mg/mL. Antibiotic-resistant strains of H. ducreyi were equally susceptible to these 3 essential oils relative to non-resistant strains (p=0.409).
CONCLUSION
E. caryophyllus, C. verum and T. satureioides oils are promising alternatives to antibiotic treatment for chancroid.
背景
杜克雷嗜血杆菌是导致生殖器溃疡疾病软下疳的细菌，是艾滋病毒传播的一个辅助因素，并且它对许多抗生素具有耐药性。为了探索可能的替代治疗方法，我们测试了香精油（EOs）作为抗杜克雷嗜血杆菌抗菌剂的功效。
方法
我们使用琼脂稀释法测定了桂皮（肉桂）、丁香（ Eugenia caryophyllus）和百里香（Thymus satureioides）油对9株杜克雷嗜血杆菌的最低抑菌浓度（MIC）。我们还通过将MIC平板上的菌液转接至不含香精油的新鲜琼脂上，测定了每种油的最低致死浓度。对于这两项测试，我们使用双向方差分析来评估耐药菌株相对于非耐药菌株对这些油的敏感性是否不同。
结果
所有3种油对杜克雷嗜血杆菌均表现出优异的活性，MIC为0.05至0.52mg/mL，MLC为0.1 - 0.5mg/mL。杜克雷嗜血杆菌的耐药菌株相对于非耐药菌株对这3种香精油同样敏感（p = 0.409）。
结论
丁香、桂皮和百里香油有望成为软下疳抗生素治疗的替代方法。
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