光固化模式对树脂基表面密封剂细胞毒性的影响。
Influence of light-curing mode on the cytotoxicity of resin-based surface sealants.
机构信息
Clinic for Preventive Dentistry, Periodontology and Cariology, University of Zurich, Plattenstrasse 11, 8032 Zürich, Switzerland.
出版信息
BMC Oral Health. 2014 May 6;14:48. doi: 10.1186/1472-6831-14-48.
BACKGROUND
Surface sealants have been successfully used in the prevention of erosive tooth wear. However, when multiple tooth surfaces should be sealed, the light-curing procedure is very time-consuming. Therefore, the aim of this study was to investigate whether reduced light-curing time (while maintaining similar energy density) has an influence on resin-based surface sealant cytotoxicity.
METHODS
Bovine dentine discs were treated as follows: group 1: untreated, groups 2-5: Seal&Protect and groups 6-9: experimental sealer. Groups 2 and 6 were light-cured (VALO LED light-curing device) for 40 s (1000 mW/cm2), groups 3 and 7 for 10 s (1000 mW/cm2), groups 4 and 8 for 7 s (1400 mW/cm2) and groups 5 and 9 for 3 s (3200 mW/cm2). Later, materials were extracted in culture medium for 24 h, and released lactate dehydrogenase (LDH) activity as a measure of cytotoxicity was determined photometrically after cells (dental pulp cells and gingival fibroblasts) were exposed to the extracts for 24 h. Three independent experiments, for both sample preparation and cytotoxicity testing, were performed.
RESULTS
Overall, lowest cytotoxicity was observed for the unsealed control group. No significant influence of light-curing settings on the cytotoxicity was observed (p = 0.537 and 0.838 for pulp cells and gingival fibroblasts, respectively). No significant difference in the cytotoxicity of the two sealants was observed after light-curing with same light-curing settings (group 2 vs. 6, 3 vs. 7, 4 vs. 8 and 5 vs. 9: p > 0.05, respectively).
CONCLUSIONS
Shortening the light-curing time, while maintaining constant energy density, resulted in no higher cytotoxicity of the investigated sealants.
背景
表面密封剂已成功用于预防侵蚀性牙齿磨损。然而，当需要密封多个牙面时，光固化过程非常耗时。因此，本研究旨在探讨减少光固化时间（同时保持相似的能量密度）是否会对基于树脂的表面密封剂的细胞毒性产生影响。
方法
牛牙本质圆盘如下处理：组 1：未处理，组 2-5：Seal&Protect，组 6-9：实验密封剂。组 2 和 6 用 VALO LED 光固化设备光固化 40 s（1000 mW/cm2），组 3 和 7 光固化 10 s（1000 mW/cm2），组 4 和 8 光固化 7 s（1400 mW/cm2），组 5 和 9 光固化 3 s（3200 mW/cm2）。之后，将材料在培养基中提取 24 h，暴露于提取物 24 h 后，通过光度法测定释放的乳酸脱氢酶（LDH）活性作为细胞毒性的衡量标准。进行了三个独立的实验，用于样本制备和细胞毒性测试。
结果
总体而言，未密封的对照组观察到的细胞毒性最低。光固化设置对细胞毒性没有显著影响（牙髓细胞和牙龈成纤维细胞的 p 值分别为 0.537 和 0.838）。在用相同的光固化设置光固化后，两种密封剂的细胞毒性没有显著差异（组 2 与 6、3 与 7、4 与 8 和 5 与 9：p>0.05，分别）。
结论
缩短光固化时间，同时保持恒定的能量密度，不会导致所研究的密封剂的细胞毒性增加。
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