Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA.
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA.
Virology. 2014 May;456-457:139-44. doi: 10.1016/j.virol.2014.03.012. Epub 2014 Apr 5.
To date, only a single Friend virus (FV) peptide recognized by CD4(+) T cells in FV-infected mice of the resistant H-2(b) haplotype has been described. To more thoroughly examine the repertoire of CD4(+) T cell responses in H-2(b) mice infected with this retrovirus, 18mer peptides spanning the FV gag, pol, and env coding regions with 11mer overlaps were synthesized. The peptides were then used to stimulate whole splenocytes and purified CD4(+) T cells from FV-infected mice in an IFNγ ELISPOT assay. Nine new CD4(+) T cell epitopes were identified, 3 encoded by gag, 1 by pol, and 5 by env. The high resistance of H-2(b) mice could be related to this very broad CD4(+) T cell response against multiple peptides during FV infection.
迄今为止，仅在具有抗性 H-2(b) 单倍型的 FV 感染小鼠中描述了一种被 CD4(+) T 细胞识别的 Friend 病毒 (FV) 肽。为了更彻底地研究感染这种逆转录病毒的 H-2(b) 小鼠中 CD4(+) T 细胞反应的组成，合成了跨越 FV gag、pol 和 env 编码区的 18 个氨基酸肽，重叠 11 个氨基酸。然后，这些肽被用于刺激 IFNγ ELISPOT 测定中来自 FV 感染小鼠的全脾细胞和纯化的 CD4(+) T 细胞。鉴定了 9 个新的 CD4(+) T 细胞表位，其中 3 个由 gag 编码，1 个由 pol 编码，5 个由 env 编码。H-2(b) 小鼠的高抗性可能与 FV 感染期间针对多种肽的这种非常广泛的 CD4(+) T 细胞反应有关。
