细小病毒 B19 VLP 在支持的脂质双层中识别Globoside。

Parvovirus B19 VLP recognizes globoside in supported lipid bilayers.

机构信息

Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy, University of Gothenburg, Bruna Stråket 16, 413 45 Gothenburg, Sweden.

Department of Infectious Diseases, Sahlgrenska Academy, University of Gothenburg, Guldhedsg. 10B, 413 46 Gothenburg, Sweden.

出版信息

Virology. 2014 May;456-457:364-9. doi: 10.1016/j.virol.2014.04.004. Epub 2014 Apr 28.

DOI:10.1016/j.virol.2014.04.004

PMID:24889255

Abstract

Studies have suggested that the glycosphingolipid globoside (Gb4Cer) is a receptor for human parvovirus B19. Virus-like particles bind to Gb4Cer on thin-layer chromatograms, but a direct interaction between the virus and lipid membrane-associated Gb4Cer has been debated. Here, we characterized the binding of parvovirus B19 VP1/VP2 virus-like particles to glycosphingolipids (i) on thin-layer chromatograms (TLCs) and (ii) incorporated into supported lipid bilayers (SLBs) acting as cell-membrane mimics. The binding specificities of parvovirus B19 determined in the two systems were in good agreement; the VLP recognized both Gb4Cer and the Forssman glycosphingolipid on TLCs and in SLBs compatible with the role of Gb4Cer as a receptor for this virus.

摘要

研究表明，糖鞘脂类Globoside (Gb4Cer) 是人类细小病毒 B19 的受体。病毒样颗粒在薄层色谱上与 Gb4Cer 结合，但病毒与脂质膜相关的 Gb4Cer 之间的直接相互作用一直存在争议。在这里，我们描述了细小病毒 B19 VP1/VP2 病毒样颗粒与糖脂（i）在薄层色谱（TLCs）上和（ii）整合到作为细胞膜模拟物的支撑脂质双层（SLBs）中的结合。在这两种系统中确定的细小病毒 B19 的结合特异性非常吻合；VLP 在 TLC 和 SLB 上都识别 Gb4Cer 和 Forssman 糖脂，这与 Gb4Cer 作为该病毒受体的作用一致。