College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China.
Molecule Biology Laboratory of Zhengzhou Normal University, Zhengzhou, Henan, China.
Microbiol Spectr. 2024 Jun 4;12(6):e0391423. doi: 10.1128/spectrum.03914-23. Epub 2024 May 14.
Porcine parvovirus (PPV) is one of the most important pathogens that cause reproductive failure in pigs. However, the pathogenesis of PPV infection remains unclear. Proteomics is a powerful tool to understand the interaction between virus and host cells. In the present study, we analyzed the proteomics of PPV-infected PK-15 cells. A total of 32 and 345 proteins were differentially expressed at the early and replication stages, respectively. Subsequent gene ontology annotation and Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed these differentially expressed proteins were significantly enriched in pathways including toll-like receptor signaling pathway, tumor necrosis factor signaling pathway, and viral carcinogenesis. The expression of poly (rC) binding protein 1 (PCBP1) was observed to decrease after PPV infection. Overexpressed or silenced PCBP1 expression inhibited or promoted PPV infection. Our studies established a foundation for further exploration of the multiplication mechanism of PPV.
Porcine parvovirus (PPV) is a cause of reproductive failure in the swine industry. Our knowledge of PPV remains limited, and there is no effective treatment for PPV infection. Proteomics of PPV-infected PK-15 cells was conducted to identify differentially expressed proteins at 6 hours post-infection (hpi) and 36 hpi. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that various pathways participate in PPV infection. Poly (rC) binding protein 1 was confirmed to inhibit PPV replication, which provided potential targets for anti-PPV infection. Our findings improve the understanding of PPV infection and pave the way for future research in this area.
猪细小病毒（PPV）是导致猪繁殖失败的最重要病原体之一。然而，PPV 感染的发病机制尚不清楚。蛋白质组学是了解病毒与宿主细胞相互作用的有力工具。在本研究中，我们分析了 PPV 感染 PK-15 细胞的蛋白质组学。在早期和复制阶段，分别有 32 种和 345 种蛋白质表达差异。随后的基因本体注释和京都基因与基因组百科全书富集分析表明，这些差异表达蛋白显著富集在 Toll 样受体信号通路、肿瘤坏死因子信号通路和病毒致癌等途径中。PPV 感染后发现多聚（rC）结合蛋白 1（PCBP1）的表达下降。过表达或沉默 PCBP1 表达可抑制或促进 PPV 感染。我们的研究为进一步探索 PPV 的增殖机制奠定了基础。
猪细小病毒（PPV）是猪养殖业繁殖失败的原因。我们对 PPV 的了解仍然有限，并且没有有效的 PPV 感染治疗方法。进行了 PPV 感染 PK-15 细胞的蛋白质组学研究，以鉴定感染后 6 小时（hpi）和 36 hpi 时的差异表达蛋白。基因本体和京都基因与基因组百科全书富集分析表明，各种途径参与了 PPV 感染。多聚（rC）结合蛋白 1被证实可抑制 PPV 复制，为抗 PPV 感染提供了潜在的靶标。我们的发现提高了对 PPV 感染的认识，为该领域的未来研究铺平了道路。
