用于核靶向的生物工程化丝基因递送系统。
Bioengineered silk gene delivery system for nuclear targeting.
作者信息
Yigit Sezin, Tokareva Olena, Varone Antonio, Georgakoudi Irene, Kaplan D L
机构信息
Department of Chemistry, Tufts University, 62 Talbot Avenue, Medford, MA 02155, USA.
出版信息
Macromol Biosci. 2014 Sep;14(9):1291-8. doi: 10.1002/mabi.201400113. Epub 2014 May 30.
Abstract
Gene delivery research has gained momentum with the use of lipophilic vectors that mimic viral systems to increase transfection efficiency. Maintaining cell viability with these systems remains a major challenge. Therefore, biocompatible biopolymers that are designed by combining non-immunological viral mimicking components with suitable carrier are explored to address these limitations. In the present study, dragline silk recombinant proteins are modified with DNA condensing units and the proton sponge endosomal escape pathway is utilized for enhanced delivery. Transfection efficiency in a COS-7 cell line is enhanced compared to lipofectamine and polyethyleneimine (PEI), as is cell viability.
摘要
随着亲脂性载体的使用，基因递送研究获得了动力，这些载体模仿病毒系统以提高转染效率。使用这些系统维持细胞活力仍然是一项重大挑战。因此，人们探索通过将非免疫性病毒模拟成分与合适的载体相结合来设计生物相容性生物聚合物，以解决这些局限性。在本研究中，牵索丝重组蛋白用DNA凝聚单元进行修饰，并利用质子海绵内体逃逸途径来增强递送。与脂质体转染试剂和聚乙烯亚胺（PEI）相比，COS-7细胞系中的转染效率提高了，细胞活力也提高了。
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