Jensen Annie, Karottki Dorina Gabriela, Christensen Jannie Marie, Bønløkke Jakob Hjort, Sigsgaard Torben, Glasius Marianne, Loft Steffen, Møller Peter
Department of Public Health, Section of Environmental Health, University of Copenhagen, Denmark.
Environ Mol Mutagen. 2014 Oct;55(8):652-61. doi: 10.1002/em.21877. Epub 2014 Jun 3.
Exposure to particles from combustion of wood is associated with respiratory symptoms, whereas there is limited knowledge about systemic effects. We investigated effects on systemic inflammation, oxidative stress and DNA damage in humans who lived in a reconstructed Viking Age house, with indoor combustion of wood for heating and cooking. The subjects were exposed to high indoor concentrations of PM2.5 (700-3,600 µg/m(3)), CO (10.7-15.3 ppm) and NO2 (140-154 µg/m(3)) during a 1-week stay. Nevertheless, there were unaltered levels of genotoxicity, determined as DNA strand breaks and formamidopyrimidine DNA glycosylase and oxoguanine DNA glycosylase 1 sensitive sites in peripheral blood mononuclear cells. There were also unaltered expression levels of OGG1, HMOX1, CCL2, IL8, and TNF levels in leukocytes. In serum, there were unaltered levels of C-reactive protein, IL6, IL8, TNF, lactate dehydrogenase, cholesterol, triglycerides, and high-density lipoproteins. The wood smoke exposure was associated with decreased serum levels of sICAM-1, and a tendency to decreased sVCAM-1 levels. There was a minor increase in the levels of circulating monocytes expressing CD31, whereas there were unaltered expression levels of CD11b, CD49d, and CD62L on monocytes after the stay in the house. In conclusion, even a high inhalation exposure to wood smoke was associated with limited systemic effects on markers of oxidative stress, DNA damage, inflammation, and monocyte activation.
接触木材燃烧产生的颗粒物与呼吸道症状有关，而关于其对全身影响的了解有限。我们调查了居住在一座重建的维京时代房屋中的人，该房屋使用木材进行室内取暖和烹饪，这对他们全身炎症、氧化应激和DNA损伤的影响。受试者在为期1周的居住期间暴露于室内高浓度的PM2.5（700 - 3600 µg/m³）、CO（10.7 - 15.3 ppm）和NO2（140 - 154 µg/m³）中。然而，外周血单核细胞中的遗传毒性水平未发生改变，遗传毒性通过DNA链断裂以及甲酰胺嘧啶DNA糖基化酶和氧代鸟嘌呤DNA糖基化酶1敏感位点来确定。白细胞中OGG1、HMOX1、CCL2、IL8和TNF的表达水平也未发生改变。血清中C反应蛋白、IL6、IL8、TNF、乳酸脱氢酶、胆固醇、甘油三酯和高密度脂蛋白的水平未发生改变。木材烟雾暴露与血清中sICAM - 1水平降低以及sVCAM - 1水平有降低趋势有关。表达CD31的循环单核细胞水平略有增加，而在居住在该房屋后，单核细胞上CD11b、CD49d和CD62L的表达水平未发生改变。总之，即使高吸入暴露于木材烟雾，对氧化应激、DNA损伤、炎症和单核细胞活化标志物的全身影响也有限。
