Section of Environmental Health, Department of Public Health, University of Copenhagen, Copenhagen, Denmark.
Part Fibre Toxicol. 2012 Mar 27;9:7. doi: 10.1186/1743-8977-9-7.
Exposure to wood smoke is associated with respiratory symptoms, whereas knowledge on systemic effects is limited. We investigated effects on systemic inflammation, oxidative stress and microvascular function (MVF) after controlled wood smoke exposure.
In a randomised, double-blinded, cross-over study 20 non-smoking atopic subjects were exposed at rest to 14, 220, or 354 μg/m3 of particles from a well-burning modern wood stove for 3 h in a climate controlled chamber with 2 week intervals. We investigated the level of oxidatively damaged DNA, inflammatory markers and adhesion molecules before and 0, 6 and 20 h after exposure. Six h after exposure we measured MVF non-invasively by digital peripheral artery tonometry following arm ischemia.
The MVF score was unaltered after inhalation of clean air (1.58 ± 0.07; mean ± SEM), low (1.51 ± 0.07) or high (1.61 ± 0.09) concentrations of wood smoke particles in atopic subjects, whereas unexposed non-atopic subjects had higher score (1.91 ± 0.09). The level of oxidatively damaged DNA, mRNA of ITGAL, CCL2, TNF, IL6, IL8, HMOX1, and OGG1 and surface marker molecules ICAM1, ITGAL and L-selectin in peripheral blood mononuclear cells were not affected by inhalation of wood smoke particles.
Exposure to wood smoke had no effect on markers of oxidative stress, DNA damage, cell adhesion, cytokines or MVF in atopic subjects.
接触木柴烟雾与呼吸道症状有关，而对系统影响的了解有限。我们研究了受控木柴烟雾暴露后对全身炎症、氧化应激和微血管功能（MVF）的影响。
在一项随机、双盲、交叉研究中，20 名非吸烟的特应性受试者在 2 周的间隔内，在气候控制室内，以 14、220 或 354μg/m3 的颗粒浓度，从一个燃烧良好的现代木炉中休息时暴露于 3 小时。我们在暴露前和暴露后 0、6 和 20 小时，调查了氧化损伤 DNA、炎症标志物和粘附分子的水平。暴露 6 小时后，我们通过手臂缺血后的数字外周动脉张力测定法，非侵入性地测量了 MVF。
在特应性受试者中，吸入清洁空气（1.58±0.07；平均值±SEM）、低（1.51±0.07）或高（1.61±0.09）浓度的木柴烟雾颗粒后，MVF 评分没有改变，而未暴露的非特应性受试者的评分较高（1.91±0.09）。外周血单核细胞中氧化损伤 DNA、ITGAL、CCL2、TNF、IL6、IL8、HMOX1 和 OGG1 的 mRNA 以及细胞表面标记分子 ICAM1、ITGAL 和 L-选择素的水平不受吸入木柴烟雾颗粒的影响。
在特应性受试者中，暴露于木柴烟雾对氧化应激、DNA 损伤、细胞粘附、细胞因子或 MVF 的标志物没有影响。
