Hardy Jonathan I, Hendricks Anke, Loeffler Anette, Chang Yu-Mei, Verheyen Kristien L, Garden Oliver A, Bond Ross
Department of Clinical Science and Services and, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire, AL9 7TA, UK.
Vet Dermatol. 2014 Oct;25(5):447-e70. doi: 10.1111/vde.12137. Epub 2014 Jun 1.
Despite conflicting data on their utility and no reports on interlaboratory reproducibility, serum food-specific antibodies are commonly assayed in first-opinion canine practice.
HYPOTHESIS/OBJECTIVES: To determine both the variability of test results between two laboratories and the frequencies and magnitudes of food reactivity in dogs of different disease status.
Sera were obtained from eight dogs with cutaneous adverse food reaction (Group A), 22 with nonfood-induced atopic dermatitis (Group B), 30 with an allergic/inflammatory phenotype (Group C), 12 with miscellaneous skin diseases (Group D) and nine healthy dogs (Group E).
Paired sera were submitted to two laboratories (A and B) for assays of food-specific IgE and IgG antibodies.
Numbers of positive IgE and IgG tests determined by each laboratory in Groups A, B, D and E were comparable (Group C not included). Significant differences in the magnitude of IgE reactivity between groups for each allergen were seen only for lamb (Laboratory A, P = 0.003); lamb reactivity in Group D exceeded Group E (P = 0.004) but was comparable between all other groups. Agreement (kappa statistic) between the two laboratories' tests was 'moderate' for one antigen (potato IgE), 'fair' for four (corn IgE, rice IgE and IgG and soya bean IgG), 'slight' for eight (six IgE and two IgG) and 'less than chance' for the remaining six antigens (three IgE and three IgG).
These laboratories' tests appear to have dubious predictive clinical utility because they neither correlate nor distinguish between dogs of different disease status.
尽管关于血清食物特异性抗体的效用数据相互矛盾，且尚无关于实验室间可重复性的报告，但在初诊犬科医疗实践中，血清食物特异性抗体检测仍很常见。
假设/目的：确定两个实验室之间检测结果的变异性，以及不同疾病状态犬类食物反应性的频率和程度。
从八只患有皮肤食物不良反应的犬（A组）、22只患有非食物性特应性皮炎的犬（B组）、30只具有过敏/炎症表型的犬（C组）、12只患有其他皮肤病的犬（D组）和九只健康犬（E组）采集血清。
将配对血清送交两个实验室（A和B）检测食物特异性IgE和IgG抗体。
A、B、D和E组中，每个实验室检测出的IgE和IgG阳性结果数量具有可比性（C组未纳入）。仅在羊肉过敏原方面，各实验室检测的IgE反应程度在不同组间存在显著差异（实验室A，P = 0.003）；D组对羊肉的反应性超过E组（P = 0.004），但在所有其他组间具有可比性。两个实验室检测结果的一致性（kappa统计量），对于一种抗原（马铃薯IgE）为“中等”，对于四种抗原（玉米IgE、大米IgE和IgG以及大豆IgG）为“一般”，对于八种抗原（六种IgE和两种IgG）为“轻微”，对于其余六种抗原（三种IgE和三种IgG）为“低于随机概率”。
这些实验室的检测在临床预测方面的效用似乎存疑，因为它们既不能区分不同疾病状态的犬类，检测结果也不具有相关性。
