Department of Life Science, Institute of Nano Science and Technology, and Research Institute for Natural Sciences, Hanyang University, Seoul, 133-791, Republic of Korea.
Mass Spectrom Rev. 2015 Mar-Apr;34(2):237-47. doi: 10.1002/mas.21437. Epub 2014 Jun 2.
Bio-conjugated nanoparticles have emerged as novel molecular probes in nano-biotechnology and nanomedicine and chemical analyses of their surfaces have become challenges. The time-of-flight (TOF) secondary ion mass spectrometry (SIMS) has been one of the most powerful surface characterization techniques for both nanoparticles and biomolecules. When combined with various nanoparticle-based signal enhancing strategies, TOF-SIMS can probe the functionalization of nanoparticles as well as their locations and interactions in biological systems. Especially, nanoparticle-based SIMS is an attractive approach for label-free drug screening because signal-enhancing nanoparticles can be designed to directly measure the enzyme activity. The chemical-specific imaging analysis using SIMS is also well suited to screen nanoparticles and nanoparticle-biomolecule conjugates in complex environments. This review presents some recent applications of nanoparticle-based TOF-SIMS to the chemical analysis of complex biological systems.
生物共轭纳米粒子作为纳米生物技术和纳米医学中的新型分子探针已经出现，对其表面的化学分析已成为一项挑战。飞行时间（TOF）二次离子质谱（SIMS）是纳米粒子和生物分子最强大的表面特性分析技术之一。当与各种基于纳米粒子的信号增强策略结合使用时，TOF-SIMS 可以探测纳米粒子的功能化及其在生物系统中的位置和相互作用。特别是，基于纳米粒子的 SIMS 是一种用于无标记药物筛选的有吸引力的方法，因为可以设计信号增强纳米粒子来直接测量酶活性。使用 SIMS 进行的化学特异性成像分析也非常适合在复杂环境中筛选纳米粒子和纳米粒子-生物分子缀合物。本综述介绍了基于纳米粒子的 TOF-SIMS 在复杂生物系统的化学分析中的一些最新应用。
