Bao Zhiyao, Zhang Qiurui, Wan Huanying, He Ping, Zhou Xin, Zhou Min
Department of Respiratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Department of Respiratory Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai 200080, China.
Department of Respiratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Chin Med J (Engl). 2014;127(11):2117-20.
Idiopathic pulmonary fibrosis (IPF) is a progressive diffuse parenchymal disease with a poor prognosis. A variety of cytokines and chemokines are involved in its pathophysiology. The aim of this study was to evaluate the clinical features in IPF patients with the expression of suppressor of cytokine signaling 1 (SOCS-1), which acts as a negative regulator of cytokine signaling.
IPF patients (n = 20) and healthy controls (n = 16) were included in this study. The expression of SOCS-1 was analyzed in peripheral blood mononuclear cells (PBMC) of subjects using RT-PCR. Interleukin 4 (IL-4), transforming growth factor β1 (TGF-β1) and type I collagen expression were also analyzed in each individual using enzyme-linked immunosorbent assay (ELISA). The clinical characteristics of IPF patients were delineated. These results were analyzed by SPSS13.0 statistics software.
SOCS-1 mRNA expression was significantly decreased in the PBMC of IPF patients compared with healthy controls; serum levels of IL-4 and TGF-β1 were higher in IPF patients. The patients with lower expression of SOCS-1 developed lower percentage of forced vital capacity (FVC%) and DLCO/VA. A patients' SOCS-1 mRNA level was negatively correlated with serum levels of IL-4, and negatively correlated with their high-resolution computed tomography (HRCT) scores.
SOCS-1 mRNA can be detected in PBMC, and it is down-regulated in IPF patients. The expression of SOCS-1 is associated with the severity of IPF patients' symptoms, so it might be the predictor of disease severity. SOCS-1 might play an important role in IPF by reducing the expression of the T helper type 2 (Th2) cell-related cytokine IL-4.
特发性肺纤维化（IPF）是一种预后不良的进行性弥漫性实质性疾病。多种细胞因子和趋化因子参与其病理生理过程。本研究旨在评估细胞因子信号转导抑制因子1（SOCS-1）表达的IPF患者的临床特征，SOCS-1作为细胞因子信号转导的负调节因子。
本研究纳入了20例IPF患者和16例健康对照。采用逆转录聚合酶链反应（RT-PCR）分析受试者外周血单个核细胞（PBMC）中SOCS-1的表达。还采用酶联免疫吸附测定（ELISA）分析每个个体的白细胞介素4（IL-4）、转化生长因子β1（TGF-β1）和I型胶原表达。描述了IPF患者的临床特征。这些结果采用SPSS13.0统计软件进行分析。
与健康对照相比，IPF患者PBMC中SOCS-1 mRNA表达显著降低；IPF患者血清IL-4和TGF-β1水平较高。SOCS-1表达较低的患者用力肺活量（FVC%）和一氧化碳弥散量/肺泡通气量（DLCO/VA）百分比降低。患者的SOCS-1 mRNA水平与血清IL-4水平呈负相关，与高分辨率计算机断层扫描（HRCT）评分呈负相关。
PBMC中可检测到SOCS-1 mRNA，且在IPF患者中下调。SOCS-1的表达与IPF患者症状的严重程度相关，因此它可能是疾病严重程度的预测指标。SOCS-1可能通过降低2型辅助性T细胞（Th2）相关细胞因子IL-4的表达在IPF中发挥重要作用。
