Gáspár Krisztián, Baráth Sándor, Nagy Georgina, Mócsai Gábor, Gyimesi Edit, Szodoray Péter, Irinyi Beatrix, Zeher Margit, Remenyik Éva, Szegedi Andrea
Department of Dermatology, University of Debrecen, Medical Centre, Debrecen, Nagyerdei krt 98, Debrecen, Hungary.
Acta Derm Venereol. 2015 Feb;95(2):151-5. doi: 10.2340/00015555-1882.
Our aim was to assess whether the presence of highly active effector T cells in atopic dermatitis (AD) is associated with changes in the number and/or function of regulatory T cells (Tregs). Flow cytometry was utilised to determine the percentage of CD4+ CD25bright CD127-/low FOXP3+ and skin-homing CLA+ CD4+ CD25bright FOXP3+ Tregs in healthy controls and AD patients. The correlation between disease severity and Treg percentages was estimated. Treg suppressor activity and cell proliferation were measured after T-cell stimulation. Significantly increased percentages of Tregs were found in AD patients compared to healthy individuals, and significant correlation between the frequency of Tregs and disease severity was also detected. The otherwise normal suppressor activity of Tregs decreased in the presence of Staphylococcus enterotoxin B (SEB). In conclusion, the continuous presence of SEB can trigger an acquired functional impairment of Tregs in AD patients and the correlation between the increased frequency of Tregs and disease severity supports their important role in AD pathogenesis.
我们的目的是评估特应性皮炎（AD）中高活性效应T细胞的存在是否与调节性T细胞（Tregs）数量和/或功能的变化相关。采用流式细胞术测定健康对照者和AD患者中CD4+ CD25bright CD127-/low FOXP3+以及皮肤归巢CLA+ CD4+ CD25bright FOXP3+ Tregs的百分比。评估疾病严重程度与Tregs百分比之间的相关性。在T细胞刺激后测量Tregs的抑制活性和细胞增殖。与健康个体相比，AD患者中Tregs的百分比显著增加，并且还检测到Tregs频率与疾病严重程度之间存在显著相关性。在存在金黄色葡萄球菌肠毒素B（SEB）的情况下，Tregs原本正常的抑制活性降低。总之，SEB的持续存在可引发AD患者Tregs获得性功能损害，Tregs频率增加与疾病严重程度之间的相关性支持了它们在AD发病机制中的重要作用。
