Department of Endocrinology, Erciyes University Medical School, 38039 Kayseri, Turkey.
Department of Medical Biology, Erciyes University Medical School, 38039 Kayseri, Turkey.
Int J Endocrinol. 2014;2014:514891. doi: 10.1155/2014/514891. Epub 2014 Apr 10.
Sheehan's Syndrome (SS) is defined as pituitary hormone deficiency due to ischemic infarction of the pituitary gland as a result of massive postpartum uterine hemorrhage. Herein, we aimed to investigate the roles of Factor II (G20210A), Factor V (G1691A), MTHFR (C677T and A1298C), PAI-1 4G/5G, and TNF- α (-308 G > A) gene polymorphisms in the etiopathogenesis of SS. Venous blood samples were obtained from 53 cases with SS and 43 healthy women. Standard methods were used to extract the genomic DNAs. Factor II (G20210A), Factor V (G1691A), and MTHFR (C677T and A1298C) polymorphisms were identified by real-time PCR. PAI-1 4G/5G and TNF- α (-308 G > A) gene polymorphisms were detected with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods. According to statistical analysis, none of the polymorphisms were found to be significantly higher in the SS group compared to the control group. Hence, we suggest that genetic factors other than Factor II, Factor V, MTHFR, PAI-1, and TNF- α gene polymorphisms should be researched in the etiopathogenesis of SS.
希恩氏综合征（SS）定义为由于大量产后子宫出血导致的垂体缺血性梗死导致的垂体激素缺乏。在此，我们旨在研究因子 II（G20210A）、因子 V（G1691A）、MTHFR（C677T 和 A1298C）、PAI-1 4G/5G 和 TNF-α（-308 G > A）基因多态性在 SS 发病机制中的作用。采集了 53 例 SS 患者和 43 例健康女性的静脉血样本。采用标准方法提取基因组 DNA。采用实时 PCR 鉴定因子 II（G20210A）、因子 V（G1691A）和 MTHFR（C677T 和 A1298C）多态性。用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）方法检测 PAI-1 4G/5G 和 TNF-α（-308 G > A）基因多态性。根据统计分析，SS 组与对照组相比，没有一种多态性明显升高。因此，我们认为 SS 的发病机制除了因子 II、因子 V、MTHFR、PAI-1 和 TNF-α 基因多态性之外，还应该研究其他遗传因素。
