Rodríguez-Priego M Esther, Bolaños-Carmona M Victoria, González-López Santiago, Rodríguez-Navarro Alejandro, Sánchez-Sánchez M Purificación
J Adhes Dent. 2014 Aug;16(4):347-56. doi: 10.3290/j.jad.a32070.
The purpose of this study was to test the hypothesis that the pulp medication ferric sulphate (FS) may remain on primary dentin, affecting its microchemical structure and the bond strength of adhesives to primary dentin.
The effects of FS and pre-bonding conditioning (37% phosphoric acid [PA] for 15 s or a self-etching primer, with or without the use of 2% chlorhexidine [CHX]) on FS-treated primary dentin were characterized using optical microscopy with Masson's and Perls' stains, variable-pressure SEM/energy-dispersive x-ray spectroscopy (VP-SEM/EDS), Fourier transform infrared spectroscopy (FT-IR), and x-ray diffraction. Ferric sulphate was applied for 30 s or 1 h for microchemical analysis. The adhesive interface and the bond strength were studied by VP-SEM/EDS and the μTBS test (1 mm2 bars, crosshead speed 0.5 mm/min), respectively. The study groups were: etch-and-rinse (E&R, Excite) adhesive (group 1); FS+E&R (group 2); FS+CHX+E&R (group 3); self-etching (SE, Adper Scotchbond) adhesive (group 4); FS+SE (group 5); FS+CHX+SE (group 6).
Ferric sulphate application produced demineralization, gypsum formation, and adsorption of Fe on the dentin surface, and it modified the collagen structure of primary dentin. There were no effects of FS on bond strength of the tested etch-and-rinse adhesive system. FS slightly reduced the bond strength between the primary dentin and the SE adhesive, and the values were not restored by CHX (group 4 = 17.58 ± 5.52 MPa > group 5 = 14.26 ± 7.08 MPa = group 6 = 13.96 ± 4.87 MPa).
Ferric sulphate alters the microchemical structure of primary dentin and can impair the adhesive strength of some self-etching adhesives.
本研究旨在验证以下假设：牙髓用药硫酸铁（FS）可能残留在乳牙本质上，影响其微化学结构以及黏结剂与乳牙本质的黏结强度。
使用马松氏染色法和派若斯染色法的光学显微镜、可变压力扫描电子显微镜/能量色散X射线光谱仪（VP-SEM/EDS）、傅里叶变换红外光谱仪（FT-IR）以及X射线衍射，对FS和预黏结预处理（37%磷酸[PA]处理15秒或使用自酸蚀底漆，使用或不使用2%洗必泰[CHX]）对经FS处理的乳牙本质的影响进行表征。硫酸铁应用30秒或1小时用于微化学分析。分别通过VP-SEM/EDS和微拉伸黏结强度测试（1平方毫米试条，横梁速度0.5毫米/分钟）研究黏结界面和黏结强度。研究组包括：酸蚀冲洗（E&R，Excite）黏结剂（第1组）；FS+E&R（第2组）；FS+CHX+E&R（第3组）；自酸蚀（SE，Adper Scotchbond）黏结剂（第4组）；FS+SE（第5组）；FS+CHX+SE（第6组）。
应用硫酸铁会导致脱矿、石膏形成以及铁在牙本质表面的吸附，并改变乳牙本质的胶原结构。FS对测试的酸蚀冲洗黏结剂系统的黏结强度没有影响。FS略微降低了乳牙本质与SE黏结剂之间的黏结强度，且CHX未能恢复该数值（第4组=17.58±5.52兆帕>第5组=14.26±7.08兆帕=第6组=13.96±4.87兆帕）。
硫酸铁会改变乳牙本质的微化学结构，并可能损害某些自酸蚀黏结剂的黏结强度。
