Mostaço-Guidolin Leila B, Kohlenberg Elicia K, Smith Michael, Hewko Mark, Major Arkady, Sowa Michael G, Ko Alex C-T
National Research Council Canada , Medical Devices Portfolio, 435 Ellice Avenue, Winnipeg, MB, Canada R3B 1Y6.
Anal Chem. 2014 Jul 1;86(13):6346-54. doi: 10.1021/ac5005635. Epub 2014 Jun 18.
Quantification of atherosclerosis has been a challenging task owing to its complex pathology. In this study, we validated a quantitative approach for assessing atherosclerosis progression in a rabbit model using a numerical matrix, optical index for plaque burden, derived directly from the nonlinear optical microscopic images captured on the atherosclerosis-affected blood vessel. A positive correlation between this optical index and the severity of atherosclerotic lesions, represented by the age of the rabbits, was established based on data collected from 21 myocardial infarction-prone Watanabe heritable hyperlipidemic rabbits with age ranging between new-born and 27 months old. The same optical index also accurately identified high-risk locations for atherosclerotic plaque formation along the entire aorta, which was validated by immunohistochemical fluorescence imaging.
由于动脉粥样硬化的病理过程复杂，对其进行量化一直是一项具有挑战性的任务。在本研究中，我们验证了一种定量方法，该方法使用数值矩阵和斑块负荷光学指数来评估兔模型中的动脉粥样硬化进展，该光学指数直接从受动脉粥样硬化影响的血管上捕获的非线性光学显微镜图像中得出。基于从21只易患心肌梗死的渡边遗传性高脂血症兔收集的数据（年龄范围从新生到27个月），建立了该光学指数与以兔龄表示的动脉粥样硬化病变严重程度之间的正相关关系。相同的光学指数还准确地识别了沿整个主动脉形成动脉粥样硬化斑块的高危位置，这通过免疫组织化学荧光成像得到了验证。
