National Research Council Canada, Institute for Biodiagnostics, 435 Ellice Avenue, Winnipeg, Manitoba, Canada R3B 1Y6.
J Biomed Opt. 2010 Mar-Apr;15(2):020501. doi: 10.1117/1.3353960.
Label-free imaging of bulk arterial tissue is demonstrated using a multimodal nonlinear optical microscope based on a photonic crystal fiber and a single femtosecond oscillator operating at 800 nm. Colocalized imaging of extracellular elastin fibers, fibrillar collagen, and lipid-rich structures within aortic tissue obtained from atherosclerosis-prone myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbits is demonstrated through two-photon excited fluorescence, second harmonic generation, and coherent anti-Stokes Raman scattering, respectively. These images are shown to differentiate healthy arterial wall, early atherosclerotic lesions, and advanced plaques. Clear pathological changes are observed in the extracellular matrix of the arterial wall and correlated with progression of atherosclerotic disease as represented by the age of the WHHLMI rabbits.
利用基于光子晶体光纤和单频飞秒振荡器（工作波长 800nm）的多模非线性光学显微镜，实现了对大体积动脉组织的无标记成像。通过双光子激发荧光、二次谐波产生和相干反斯托克斯拉曼散射，分别对从动脉粥样硬化易患、心肌梗死易患的 Watanabe 遗传性高脂血症（WHHLMI）兔主动脉组织中获得的细胞外弹性纤维、纤维状胶原和富含脂质的结构进行共定位成像。这些图像可区分健康的动脉壁、早期动脉粥样硬化病变和晚期斑块。在动脉壁的细胞外基质中观察到明显的病理变化，并与 WHHLMI 兔的年龄所代表的动脉粥样硬化疾病的进展相关。
