State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University , Beijing 100084, China.
ACS Appl Mater Interfaces. 2014 Jun 25;6(12):9733-41. doi: 10.1021/am502170s. Epub 2014 Jun 11.
Tuning the material properties in order to control the cellular behavior is an important issue in tissue engineering. It is now well-established that the surface chemistry can affect cell adhesion, proliferation, and differentiation. In this study, plasma polymerization, which is an appealing method for surface modification, was employed to generate surfaces with different chemical compositions. Allylamine (AAm), acrylic acid (AAc), 1,7-octadiene (OD), and ethanol (ET) were used as precursors for plasma polymerization in order to generate thin films rich in amine (-NH2), carboxyl (-COOH), methyl (-CH3), and hydroxyl (-OH) functional groups, respectively. The surface chemistry was characterized by X-ray photoelectron spectroscopy (XPS), the wettability was determined by measuring the water contact angles (WCA) and the surface topography was imaged by atomic force microscopy (AFM). The effects of surface chemical compositions on the behavior of human adipose-derive stem cells (hASCs) were evaluated in vitro: Cell Count Kit-8 (CCK-8) analysis for cell proliferation, F-actin staining for cell morphology, alkaline phosphatase (ALP) activity analysis, and Alizarin Red S staining for osteogenic differentiation. The results show that AAm-based plasma-polymerized coatings can promote the attachment, spreading, and, in turn, proliferation of hASCs, as well as promote the osteogenic differentiation of hASCs, suggesting that plasma polymerization is an appealing method for the surface modification of scaffolds used in bone tissue engineering.
调整材料性能以控制细胞行为是组织工程中的一个重要问题。现在已经确立，表面化学可以影响细胞的黏附、增殖和分化。在这项研究中，等离子体聚合被用来生成具有不同化学成分的表面，这是一种很有吸引力的表面改性方法。丙烯胺（AAm）、丙烯酸（AAc）、1,7-辛二烯（OD）和乙醇（ET）分别被用作等离子体聚合的前体，以生成分别富含胺（-NH2）、羧基（-COOH）、甲基（-CH3）和羟基（-OH）官能团的薄膜。表面化学性质通过 X 射线光电子能谱（XPS）进行表征，通过测量水接触角（WCA）来确定润湿性，表面形貌通过原子力显微镜（AFM）成像。体外评估表面化学成分对人脂肪源性干细胞（hASCs）行为的影响：细胞计数试剂盒-8（CCK-8）分析细胞增殖、F-肌动蛋白染色观察细胞形态、碱性磷酸酶（ALP）活性分析和茜素红 S 染色进行成骨分化。结果表明，AAm 基等离子体聚合涂层可以促进 hASCs 的附着、铺展，进而促进其增殖，并促进 hASCs 的成骨分化，表明等离子体聚合是骨组织工程中支架表面改性的一种很有吸引力的方法。
