Arkansas Biosciences Institute and College of Agriculture and Technology, Arkansas State University, PO Box 639, Jonesboro, AR 72401, USA.
Microb Cell Fact. 2014 Jun 3;13:79. doi: 10.1186/1475-2859-13-79.
Green microalgae represent a renewable natural source of vitamin E. Its most bioactive form is the naturally occurring RRR-α-tocopherol which is biosynthesized in photosynthetic organisms as a single stereoisomer. It is noteworthy that the natural and synthetic α-tocopherols are different biomolecular entities. This article focuses on RRR-α-tocopherol production in Stichococcus bacillaris strain siva2011 biomass in a bioreactor culture with methyl jasmonate (MeJa) elicitor. Additionally, a nonlinear mathematical model was used to quantitatively scale-up and predict the biomass production in a 20 L balloon bioreactor with dual variables such as time and volume.
Approximately 0.6 mg/g dry weight (DW) of RRR-α-tocopherol was enhanced in S. bacillaris strain siva2011 biomass with the MeJa 50 μL/L for 24 hrs elicitations when compared to the control. The R2 value from the nonlinear model was enhanced up to 95% when compared to the linear model which significantly improved the accuracy for estimating S. bacillaris strain siva2011 biomass production in a balloon bioreactor.
S. bacillaris strain siva2011 is a new green microalga which biosynthesizes significant amounts of RRR-α-tocopherol. Systematically validated dual variable empirical data should provide key insights to multivariable or fourth order modeling for algal biomass scale-up. This bioprocess engineering should provide valuable information for industrial production of RRR-α-tocopherol from green cells.
绿色微藻是维生素 E 的可再生天然来源。其最具生物活性的形式是天然存在的 RRR-α-生育酚，它在光合生物中作为单一立体异构体生物合成。值得注意的是，天然和合成的α-生育酚是不同的生物分子实体。本文重点研究了甲基茉莉酸（MeJa）诱导剂在生物反应器培养物中，Stichococcus bacillaris 菌株 siva2011 生物量中 RRR-α-生育酚的生产。此外，还使用非线性数学模型对 20 L 气球生物反应器中双变量（如时间和体积）的生物量生产进行了定量放大和预测。
与对照相比，用 50 μL/L MeJa 诱导 24 小时后，S. bacillaris 菌株 siva2011 生物量中 RRR-α-生育酚的含量增加了约 0.6mg/g 干重（DW）。与线性模型相比，非线性模型的 R2 值提高了 95%，这显著提高了估计气球生物反应器中 S. bacillaris 菌株 siva2011 生物量生产的准确性。
S. bacillaris 菌株 siva2011 是一种新的绿色微藻，能够生物合成大量的 RRR-α-生育酚。经过系统验证的双变量经验数据应该为藻类生物量放大的多变量或四阶建模提供关键见解。该生物工艺工程应为绿色细胞中 RRR-α-生育酚的工业生产提供有价值的信息。
