Pal Priyanka D, Dongre Prabhakar M, Chitre Arunkumar V
Department of Biophysics, University Of Mumbai, Vidyanagri Santacruz (E), Mumbai, 400098, India.
J Fluoresc. 2014 Jul;24(4):1275-84. doi: 10.1007/s10895-014-1412-1. Epub 2014 Jun 5.
Biological macromolecules evolve and function within intracellular environments that are crowded with other macromolecules. Crowding results in surprisingly large quantitative effects on both the rates and the equilibria of interactions involving macromolecules, but such interactions are commonly studied outside the cell in uncrowded buffers. The addition of high concentrations of natural and synthetic macromolecules to such buffers enables crowding to be mimicked in vitro, and should be encouraged as a routine variable to study. In this study, we propose to understand the changes in DNA character and its modulation in presence of macromolecules such as PEG with reference to binding parameters to amino acids using fluorescence enhancement.
生物大分子在充满其他大分子的细胞内环境中进化并发挥功能。拥挤对涉及大分子的相互作用的速率和平衡产生了惊人的巨大定量影响，但此类相互作用通常是在细胞外的非拥挤缓冲液中进行研究的。向此类缓冲液中添加高浓度的天然和合成大分子能够在体外模拟拥挤状态，应鼓励将其作为一个常规变量进行研究。在本研究中，我们建议通过荧光增强来了解在聚乙二醇等大分子存在的情况下DNA特性的变化及其对与氨基酸结合参数的调节作用。
