斑马鱼幼体的脊髓横切术。
Spinal cord transection in the larval zebrafish.
作者信息
Briona Lisa K, Dorsky Richard I
机构信息
Department of Neurobiology & Anatomy, University of Utah.
Department of Neurobiology & Anatomy, University of Utah;
出版信息
J Vis Exp. 2014 May 21(87):51479. doi: 10.3791/51479.
Mammals fail in sensory and motor recovery following spinal cord injury due to lack of axonal regrowth below the level of injury as well as an inability to reinitiate spinal neurogenesis. However, some anamniotes including the zebrafish Danio rerio exhibit both sensory and functional recovery even after complete transection of the spinal cord. The adult zebrafish is an established model organism for studying regeneration following spinal cord injury, with sensory and motor recovery by 6 weeks post-injury. To take advantage of in vivo analysis of the regenerative process available in the transparent larval zebrafish as well as genetic tools not accessible in the adult, we use the larval zebrafish to study regeneration after spinal cord transection. Here we demonstrate a method for reproducibly and verifiably transecting the larval spinal cord. After transection, our data shows sensory recovery beginning at 2 days post-injury (dpi), with the C-bend movement detectable by 3 dpi and resumption of free swimming by 5 dpi. Thus we propose the larval zebrafish as a companion tool to the adult zebrafish for the study of recovery after spinal cord injury.
由于损伤平面以下轴突再生不足以及无法重新启动脊髓神经发生，哺乳动物在脊髓损伤后感觉和运动功能无法恢复。然而，一些无羊膜动物，包括斑马鱼Danio rerio，即使在脊髓完全横断后仍能实现感觉和功能恢复。成年斑马鱼是研究脊髓损伤后再生的成熟模式生物，损伤后6周可实现感觉和运动恢复。为了利用透明幼体斑马鱼体内的再生过程分析以及成年斑马鱼无法使用的基因工具，我们使用幼体斑马鱼研究脊髓横断后的再生。在此，我们展示了一种可重复且可验证地横断幼体脊髓的方法。横断后，我们的数据显示损伤后2天（dpi）开始出现感觉恢复，3 dpi可检测到C形弯曲运动，5 dpi恢复自由游动。因此，我们建议将幼体斑马鱼作为成年斑马鱼的辅助工具，用于研究脊髓损伤后的恢复。
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