微小RNA-186靶向Rho相关卷曲螺旋蛋白激酶1以抑制非小细胞肺癌细胞的生长和转移。
MiR-186 targets ROCK1 to suppress the growth and metastasis of NSCLC cells.
作者信息
Cui Guanghui, Cui Mingwei, Li Yuhang, Liang Youguang, Li Weihao, Guo Haizhou, Zhao Song
机构信息
Department of Thoracic Surgery, The First Affiliated Hospital of Zhengzhou University, 450000, Zhengzhou, China.
出版信息
Tumour Biol. 2014 Sep;35(9):8933-7. doi: 10.1007/s13277-014-2168-6. Epub 2014 Jun 4.
MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in human cancers. Increasing evidence shows that deregulation of miRNAs contributes to the development and progression of human non-small cell lung cancer (NSCLC). Here, we identified miR-186 as a tumor suppressor in NSCLC, which was decreased in NSCLC. Overexpression of miR-186 significantly inhibited proliferation, migration, and invasion of NSCLC cells. In addition, Rho-associated protein kinase 1 (ROCK1) was identified as a target of miR-186 in NSCLC cells. Restoration of ROCK1 remarkably reversed the tumor-suppressive effects of miR-186 on cell proliferation, migration, and invasion in NSCLC cells. Furthermore, ROCK1 was inversely correlated with miR-186 expression in NSCLC. Collectively, our data indicate that miR-186 functions as tumor suppressor in NSCLC by targeting ROCK1.
微小RNA（miRNA）在人类癌症中可作为癌基因或肿瘤抑制因子。越来越多的证据表明，miRNA失调会促进人类非小细胞肺癌（NSCLC）的发生和发展。在此，我们鉴定出miR-186是NSCLC中的一种肿瘤抑制因子，其在NSCLC中表达降低。miR-186的过表达显著抑制了NSCLC细胞的增殖、迁移和侵袭。此外，Rho相关蛋白激酶1（ROCK1）被鉴定为NSCLC细胞中miR-186的一个靶标。ROCK1的恢复显著逆转了miR-186对NSCLC细胞增殖、迁移和侵袭的肿瘤抑制作用。此外，在NSCLC中ROCK1与miR-186的表达呈负相关。总的来说，我们的数据表明miR-186通过靶向ROCK1在NSCLC中发挥肿瘤抑制作用。
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